SOB/SOC: Super Optimal Broth

For SOB:

1L	Final [ ]	Component
5 g	0.5%	yeast extract
20 g	2.0%	tryptone
0.5 g	10 mM	NaCl
0.186 g	2 mM	KCl
2.4 g	20 mM	MgSO4 (anhydrous)

Add 800 ml of dH₂O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH₂O to a final volume 1 L. Autoclave

For SOC (=SOB+glucose):
Make SOB, except add dH₂O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% glucose.

Note: Some recipes for SOB use 10 mM MgSO₄ and 10 mM MgCl.