SOB: Super Optimal Broth

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4

Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume of 960 ml. Autoclave.

This topic: Lab > ProtocolsMediaRecipes > ProtocolsRecipesSOB
Topic revision: r2 - 2013-09-03 - AlvaroRodriguez