SOB: Super Optimal Broth
1L |
Final [ ] |
Component |
5 g |
0.5% |
yeast extract |
20 g |
2.0% |
tryptone |
0.5 g |
10 mM |
NaCl |
0.186 g |
2 mM |
KCl |
2.4 g |
20 mM |
MgSO4 |
Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H
2O to a final volume of 960 ml. Autoclave.