---+++ SOB/SOC: Super Optimal Broth *For SOB:* <br> %TABLE{}% | *1L* | *Final [ ]* | *Component* | | 5 g | 0.5% | yeast extract | | 20 g | 2.0% | tryptone | | 0.5 g | 10 mM | NaCl | | 0.186 g | 2 mM | KCl | | 2.4 g | 20 mM | MgSO<sub>4</sub> (anhydrous) | Add 800 ml of dH<sub>2</sub>O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH<sub>2</sub>O to a final volume 1 L. Autoclave *For SOC (=SOB+glucose)*:<br> Make SOB, except add dH<sub>2</sub>O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% glucose. *Note:* Some recipes for SOB use 10 mM MgSO<sub>4</sub> and 10 mM MgCl.
This topic: Lab
>
ProtocolsMediaRecipes
>
ProtocolsRecipesSOB
Topic revision: r3 - 2018-12-10 - JeffreyBarrick