---+++ SOB: Super Optimal Broth %TABLE{}% | *1L* | *Final [ ]* | *Component* | | 5 g | 0.5% | yeast extract | | 20 g | 2.0% | tryptone | | 0.5 g | 10 mM | NaCl | | 0.186 g | 2 mM | KCl | | 2.4 g | 20 mM | MgSO<sub>4</sub> | Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H<sub>2</sub>O to a final volume of 960 ml. Autoclave.
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Topic revision: r1 - 2012-01-11 - CraigBarnhart