R2A

1L 5L Component
0.5 g 2.5 g Yeast Extract
0.5 g 2.5 g Proteose Peptone No. 3
0.5 g 2.5 g Casamino Acids
0.5 g 2.5 g Dextrose
0.5 g 2.5 g Soluble Starch
0.3 g 1.5 g Sodium Pyruvate
0.3 g 1.5 g Dipotassium Phosphate
0.05 g 0.25 g Magnesium Sulfate
Add dH2O to final volume and autoclave.

If making R2A solid media also add 15 g of agar and 1 ml of 5% antifoam per liter:

This topic: Lab > ProtocolsMediaRecipes > ProtocolsRecipesR2A
Topic revision: r1 - 2011-07-29 - CraigBarnhart