MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when agar and phosphate or phosphate and glucose are autoclaved together.

500 ml 1L 1.5L Component MW
2.65 g 5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
166.7 ml 333 ml 500 ml dH2O  

Separately, prepare the agar:

500 ml 1L 1.5L Component
8 g 16 g 24 g Agar
500 µl 1 ml 1.5 ml Antifoam (5%)
166.7 ml 333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar solution. The final concentration is 0.4% w/v:

500 ml 1L 1.5L Component
2 g 4 g 6 g Glucose, arabinose, or other sugar
166.7 ml 333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)

Source: Lenski Lab Protocol

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Topic revision: r11 - 2024-10-22 - AlexaMorton