<noautolink> ---+++ MG: Minimal Glucose, aka DM: Davis Minimal *Notes: Salts, agar and sugars need to be prepared and autoclaved separately.* <br> | *1L* | *1.5L* | *Component* | *MW* | | 7 g | 10.5 g | Potassium Phosphate (dibasic, trihydrate) HK<sub>2</sub>PO<sub>4</sub> (H<sub>2</sub>O)<sub>3</sub> | MW 228.17 | | 2 g | 3 g | Potassium Phosphate (monobasic) H<sub>2</sub>KPO<sub>4</sub> | MW 136.07 | | 1 g | 1.5 g | Ammonium Sulfate (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>| MW 132.08 | | 0.5 g | 0.75 g | Sodium Citrate (trisodium, dihydrate) Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub> (H<sub>2</sub>O)<sub>2</sub>| MW 294.10 | | 333 ml | 500 ml | dH<sub>2</sub>O | | Autoclave. <br> Next, prepare the agar: <br> | *1L* | *1.5L* | *Component* | | 16 g | 24 g | Agar | | 1 ml | 1.5 ml | Antifoam (5%) | | 333 ml | 500 ml | dH<sub>2</sub>O | Autoclave. <br> Next, prepare the sugar (0.2%): <br> | *1L* | *1.5L* | *Component* | | 4 g | 6 g | Glucose (or other sugar) | | 333 ml | 500 ml | dH<sub>2</sub>O | Autoclave. <br> After autoclaving combine the contents of the three flasks together and add the following stock solutions: <br> | *1L* | *1.5L* | *Component* | *MW* | | 1.0 ml | 1.5 ml | 10% Magnesium Sulfate MgSO<sub>4</sub> | MW 120.37 | | 1.0 ml | 1.5 ml | 0.2% Thiamin (vitamin B1) | *Note* Thiamin must be syringe filtered into the media |
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Topic revision: r2 - 2011-08-03 - JeffreyBarrick