<noautolink> ---+++ MG: Minimal Glucose agar, aka DM: Davis Mingioli agar *%RED%Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when [[http://schaechter.asmblog.org/schaechter/2014/12/the-great-plate-count-anomaly-that-is-no-more.html][agar and phosphate]] or phosphate and glucose are autoclaved together.%ENDCOLOR%* | *500 ml* | *1L* | *1.5L* | *Component* | *MW* | | 2.65 g | 5.3 g | 8 g | Potassium Phosphate (dibasic) K<sub>2</sub>HPO<sub>4</sub> | MW 174.18 | | 1 g | 2 g | 3 g | Potassium Phosphate (monobasic) KH<sub>2</sub>PO<sub>4</sub> | MW 136.07 | | 0.5 g | 1 g | 1.5 g | Ammonium Sulfate (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>| MW 132.08 | | 0.25 g | 0.5 g | 0.75 g | Sodium Citrate (trisodium, dihydrate) Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub> (H<sub>2</sub>O)<sub>2</sub>| MW 294.10 | | 166.7 ml | 333 ml | 500 ml | dH<sub>2</sub>O | | Separately, prepare the agar: <br> | *500 ml* | *1L* | *1.5L* | *Component* | | 8 g | 16 g | 24 g | Agar | | 500 µl | 1 ml | 1.5 ml | Antifoam (5%) | | 166.7 ml | 333 ml | 500 ml | dH<sub>2</sub>O | *Autoclave.* <br> Next, prepare the sugar solution. The final concentration is 0.4% w/v: <br> | *500 ml* | *1L* | *1.5L* | *Component* | | 2 g | 4 g | 6 g | Glucose, arabinose, or other sugar | | 166.7 ml | 333 ml | 500 ml | dH<sub>2</sub>O | *Autoclave.* <br> After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions: <br> | *1L* | *1.5L* | *Component* | | 1.0 ml | 1.5 ml | 10% Magnesium Sulfate MgSO<sub>4</sub> (separately autoclaved stock) | | 1.0 ml | 1.5 ml | 0.2% Thiamin (vitamin B1) (filter sterilized stock) | Source: [[http://myxo.css.msu.edu/ecoli/dmagar.html][Lenski Lab Protocol]]
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Topic revision: r11 - 2024-10-22 - AlexaMorton