<noautolink> ---+++ MG: Minimal Glucose agar, aka DM: Davis Mingioli agar *%RED%Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when [[http://schaechter.asmblog.org/schaechter/2014/12/the-great-plate-count-anomaly-that-is-no-more.html][agar and phosphate]] or phosphate and glucose are autoclaved together.%ENDCOLOR%* | *500 mL* | *1.0L* | *Component* | | 2.65 g | 5.3 g | Potassium Phosphate (dibasic) K<sub>2</sub>HPO<sub>4</sub> | | 1 g | 2 g | Potassium Phosphate (monobasic) KH<sub>2</sub>PO<sub>4</sub> | | 0.5 g | 1 g | Ammonium Sulfate (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>| | 0.25 g | 0.5 g | Sodium Citrate (trisodium, dihydrate) Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub> (H<sub>2</sub>O)<sub>2</sub>| | 166.7 mL | 333 mL | dH<sub>2</sub>O | Separately, prepare the agar: <br> | *500 mL* | *1.0 L* | *Component* | | 8 g | 16 g | Agar | | 500 µL | 1 mL | Antifoam (5%) | | 166.7 mL | 333 mL | dH<sub>2</sub>O | Next, prepare the sugar solution. The final concentration is 0.4% w/v: <br> | *500 mL* | *1.0 L* | *Component* | | 2 g | 4 g | Glucose, arabinose, or other sugar | | 166.7 mL | 333 mL | dH<sub>2</sub>O | *Autoclave the 3 parts separately for a 40 minute sterilization time.* <br> After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions: <br> | *500 mL* | *1.0 L* | *Component* | | 500 µL | 1.0 ml | 10% MgSO<sub>4</sub> (separately autoclaved stock) | | 500 µL | 1.0 ml | 0.2% Thiamine (vitamin B1) (filter sterilized stock refrigerated at 4ºC) | Source: [[http://myxo.css.msu.edu/ecoli/dmagar.html][Lenski Lab Protocol]]
This topic: Lab
>
ProtocolsMediaRecipes
>
ProtocolsRecipesMinimalGlucose
Topic revision: r12 - 2025-01-09 - AlexaMorton
Copyright ©2025 Barrick Lab contributing authors. Ideas, requests, problems?
Send feedback
