MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when agar and phosphate or phosphate and glucose are autoclaved together.

500 mL 1.0L Component
2.65 g 5.3 g Potassium Phosphate (dibasic) K2HPO4
1 g 2 g Potassium Phosphate (monobasic) KH2PO4
0.5 g 1 g Ammonium Sulfate (NH4)2 SO4
0.25 g 0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2
166.7 mL 333 mL dH2O

Separately, prepare the agar:

500 mL 1.0 L Component
8 g 16 g Agar
500 µL 1 mL Antifoam (5%)
166.7 mL 333 mL dH2O

Next, prepare the sugar solution. The final concentration is 0.4% w/v:

500 mL 1.0 L Component
2 g 4 g Glucose, arabinose, or other sugar
166.7 mL 333 mL dH2O
Autoclave the 3 parts separately for a 40 minute sterilization time.

After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:

500 mL 1.0 L Component
500 µL 1.0 ml 10% MgSO4 (separately autoclaved stock)
500 µL 1.0 ml 0.2% Thiamine (vitamin B1) (filter sterilized stock refrigerated at 4ºC)

Source: Lenski Lab Protocol

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Topic revision: r12 - 2025-01-09 - AlexaMorton