| 1L | Component |
|---|---|
| 6 g | Sodium phosphate dibasic (anhydrous), Na2HPO4 |
| 3 g | Potassium phosphate monobasic, KH2PO4 |
| 0.5 g | Sodium chloride, NaCl |
| 1 g | Ammonium chloride, NH4Cl |
After media is autoclaved add the following sterile ingredients:
| 1L | Component |
|---|---|
| 1 ml | 1M Magnesium sulfate, MgSO4 |
| 1 ml | 0.1M Calcium chloride CaCl2 |
| 10 ml | 10% (w/v) glucose (or other carbon source) |
| Final composition | |
|---|---|
| 93.0 mM | Sodium (Na+) |
| 22.1 mM | Potassium (K+) |
| 18.7 mM | Ammonium (NH4) |
| 1.0 mM | Calcium (Ca2+) |
| 0.1 mM | Magnesium (Mg2+) |
| 29.2 mM | Chloride (Cl- |
| 0.1 mM | Sulfate (SO42-) |
| 42.2 mM | Phosphate (PO43-) |