---+++ M9 Minimal Media | *1L* | *Component* | | 6 g | Sodium phosphate, Na<sub>2</sub>HPO<sub>4</sub> | | 3 g | Potassium phosphate, KH<sub>2</sub>PO<sub>4</sub> | | 0.5 g | Sodium chloride, NaCl | | 1 g | Ammonium chloride, NH<sub>4</sub>Cl | Add dH<sub>2</sub>O to 1 liter. Autoclave. After media is autoclaved add the following sterile ingredients: | *1L* | *Component* | | 1 ml | 1M Magnesium sulfate, MgSO<sub>4</sub> | | 1 ml | 0.1M Calcium chloride CaCl<sub>2</sub> | | 10 ml |10% (w/v) glucose (or other carbon source) |
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Topic revision: r5 - 2012-06-04 - CraigBarnhart