---+++ M9 Minimal Make 5× M9 salts: | 64 g | Sodium phosphate (dibasic, 7 hydrate) Na<sub>2</sub>HPO<sub>4</sub>•H<sub>2</sub>O | | 15 g | Potassium phosphate (monobasic) KH<sub>2</sub>PO<sub>4</sub> | | 2.5 g | Sodium chloride, NaCl | | 5 g | Ammonium chloride NH<sub>4</sub>Cl | Add dH<sub>2</sub>O to 1 liter. Autoclave. Combine the following ingredients (all pre-sterilized): | 200 ml | 5× M9 salts | | 2 ml | 1M Magnesium sulfate MgSO<sub>4</sub> | | 0.1 ml | 1 M Calcium chloride CaCl<sub>2</sub> | | 40 ml | 10% (w/v) glucose (or other carbon source) | Add *sterile* dH<sub>2</sub>O to 1 liter. Autoclave. <br> To make M9 plates add the items above to 16g of sterilized agar. <br> If making less than 1 liter use 8g of agar per 500ml of solution. <br>
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Topic revision: r3 - 2011-06-27 - CraigBarnhart