---+++ LB: Luria-Broth Combine in a 2 L flask the following: | *1.5L* | *Component* | | 15 g | Tryptone | | 7.5 g | Yeast Extract | | 15 g | NaCl | | 24 g | Agar | | 1.5 ml | 5% Antifoam | Add dH<sub>2</sub>O to 1.5 L. Autoclave. *Note: This is _different_ from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!* To make "soft" agar for phage studies, use only 7 g agar per liter.
This topic: Lab
>
ProtocolsMediaRecipes
>
ProtocolsRecipesLysogenyBrothAgar
Topic revision: r3 - 2011-07-29 - CraigBarnhart