ProtocolsRecipesLysogenyBrothAgar < Lab

---+++ LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

|  *1.5L*  |  *Component*  |
| 15 g | Tryptone | 
| 7.5 g | Yeast Extract |
| 15 g | <nop>NaCl | 
| 24 g | Agar | 
| 1.5 ml | 5% Antifoam | 

Add dH<sub>2</sub>O to 1.5 L. Autoclave.

*Notes and variations:*
   * [[http://en.wikipedia.org/wiki/Lysogeny_broth][Common synonyms]] are Luria-Broth and Luria-Bertani medium.
   * This is the "Miller" formulation of LB with 10 g <nop>NaCl per liter (1% w/v). *Another common formulation has only 5 g <nop>NaCl per liter (0.5% w/v)!* They are NOT interchangeable.
   * If you are performing _sacB_ counter-selection, you may need to use even lower salt concentrations.
   * To make "soft" agar for phage studies, use only 7 g agar per liter.

This topic: Lab > ProtocolsMediaRecipes > ProtocolsRecipesLysogenyBrothAgar
Topic revision: r7 - 2019-06-06 - JeffreyBarrick