---+++ DM: Davis-Mingioli Growth medium used by the long-term _E. coli_ evolution experiment. | *1L* | *5L* | *Component* | *MW* | | 5.3 g | 26.7 g | Potassium Phosphate (dibasic) K<sub>2</sub>HPO<sub>4</sub> | MW 174.18 | | 2 g | 10 g | Potassium Phosphate (monobasic) KH<sub>2</sub>PO<sub>4</sub> | MW 136.07 | | 1 g | 5 g | Ammonium Sulfate (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>| MW 132.08 | | 0.5 g | 2.5 g | Sodium Citrate (trisodium, dihydrate) Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub> (H<sub>2</sub>O)<sub>2</sub>| MW 294.10 | Add dH<sub>2</sub>O to final volume and autoclave. <br> After autoclaving add the following stock solutions: <br> | *1L* | *5L* | *Component* | | 1.0 ml | 5 ml | 10% (w/v) Magnesium Sulfate <nop>MgSO<sub>4</sub> (separately autoclaved stock) | | 1.0 ml | 5 ml | 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized) | And supplement with a carbon source. If preparing DM-glucose (MW 180.16 g/mol), add this volume of *10% glucose* solution (separately autoclaved stock) to get the final concentration desired: | *1L* | *5L* | *DMX* | *[glucose] (w/v)* | *[glucose] (mg/L)* | *[glucose] (M)* | | 250µl | 1.25 ml | DM25 | 0.0025% | 25 mg/L | 139 µM | | 1 ml | 5 ml | DM100 | 0.010% | 100 mg/L | 694 µM | | 2.5 ml | 12.5 ml | DM250 | 0.025% | 250 mg/L | 1.39 µM | | 5 ml | 25 ml | DM500 | 0.05% | 500 mg/L | 2.78 mM | | 10 ml | 50 ml | DM1000 | 0.1% | 1000 mg/L | 5.55 mM | | 20 ml | 100 ml | DM2000 | 0.2% | 2000 mg/L | 11.1 mM | Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000. | *Final composition* || | 5.1 mM | Sodium (Na<sup>+</sup>) | | 75.8 mM | Potassium (K<sup>+</sup>) | | 15.2 mM | Ammonium (NH<sub>4</sub>) | | 0.83 mM | Magnesium (Mg<sup>2+</sup>) | | 8.41 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) | | 45.3 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) | | 1.7 mM | Citrate | | 139 µM | Glucose (in DM25) |
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Topic revision: r16 - 2013-06-25 - AurkoDasgupta