ProtocolsRecipes1416,4-hydroxybenzoicacidmedium < Lab

---+++ 1416:  4-hydroxybenzoic acid medium (for JJ-1b, Bacillus sp.)

|  *1L*  |   *4L*  |  *Component*  |
| 4.25 g | 17.0 g | Potassium Phosphate (dibasic) K<sub>2</sub>HPO<sub>4</sub> trihydrate |
| 1.00 g | 4.00 g | Sodium Phosphate (monobasic) NaH<sub>2</sub>PO<sub>4</sub> monohydrate |
| 2.00 g | 8.00 g | Ammonium chloride NH<sub>4</sub>Cl |
| 0.20 g | 0.40 g | Magnesium Sulfate MgSO<sub>4</sub> heptahydrate |
| 12.0 mg | 48.0 mg | Ferrous Sulfate FeSO<sub>4</sub> heptahydrate |
| 3.00 mg | 12.0 mg | Manganese Sulfate MnSO<sub>4</sub> monohydrate |
| 3.00 mg | 12.0 mg | Zinc Sulfate ZnSO<sub>4</sub> heptahydrate |
| 1.00 mg | 4.00 mg | Cobalt (II) Sulfate CoSO<sub>4</sub> |
| 0.10 g | 0.40 g | Nitrilotriacetic acid * |
| 1.00 g | 4.00 g | 4-Hydroxybenzoic acid * |

* Dissolve these components in water made alkaline with concentrated NaOH before adding other components.

Adjust medium for final pH 7.2, +/- 0.2.  Autoclave for at least 15 minutes.

If plates needed, add 15.0 g of agar per liter to the above recipe.

This topic: Lab > ProtocolsMediaRecipes > ProtocolsRecipes1416,4-hydroxybenzoicacidmedium
Topic revision: r1 - 2013-06-24 - CraigBarnhart