---+++ MOB: Mobility Media This media is useful for enhancing the mobility of ADP1, which moves across the plate by "twitching". %TABLE{}% | *1L* | *Final [ ]* | *Component* | | 5 g | 0.5% | agarose | | 5 g | 2.0% | tryptone | | 2.5 g | 10 mM | NaCl | After pouring your gel, let it set overnight at room temperature. The next day, spot with 1uL of your overnight-grown culture, leave the plate to dry for 15 minutes at room temperature, wrap in Parafilm, invert, and incubate at 30°C. You should see spreading over the next 24 hours. -- Main.JuliePerreau - 15 Dec 2016
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Topic revision: r1 - 2016-12-15 - JuliePerreau