---+ Protocol Updates Needed * General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler) * Things like performing a dilution vs eps curve to avoid swarming, sample prep, etc. * Add a page for purifying and storing HMW DNA (Isaac) * Update PCR protocol to emphasize not adding too much template (ex: miniprep). * Ideally, also include a calculation of how many cycles are needed. * Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells? * Add a page with standard plate reader settings for different fluorophores (with citations) * Also explain setting the gain! * Add a page describing tools for plotting and fitting growth curves * [[ProtocolsStandardPCR][Standard PCR]] – add troubleshooting information here? * Page with books/resources for students joining the lab? (Lucio) * Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read... * Nuke most topics under computation and many under reference (Dan) * Link all the golden gate materials (and work on general GGA materials) * PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ) * Migrate LTEE stuff to LTEE website (Jeff) * Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler) * <del>[[SoftwareList][Computation tool list]] -- Add pLannotate and OSTIR (Cameron)</del> * <del>[[LabPersonnel][Team]] – add start dates, be sure no former lab members were not transferred there.</del> * <del>Update research interests page (Jeff)</del> * <del>[[ProtocolsLearnBiocomputing][Anaconda should not be recommended]]</del> * Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s). * Add information about Lactobacillus strains in bees.
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Topic revision: r38 - 2025-03-17 - TylerDeJong