---+ Protocol Updates Needed * [[ProcedureDNASequencing][this page on DNA sequencing]] needs to be updated and be made more for the general public than to the lab * General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler) * Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc. * Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler) * Add a page for purifying and storing HMW DNA (Isaac) * Update PCR protocol to emphasize not adding too much template (ex: miniprep). * Ideally, also include a calculation of how many cycles are needed. * <del>Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?</del> * <del>Add a page with standard plate reader settings for different fluorophores (with citations)</del> * Also explain setting the gain! * Add a page describing tools for plotting and fitting growth curves * [[ProtocolsStandardPCR][Standard PCR]] – add troubleshooting information here? * Page with books/resources for students joining the lab? (Lucio) * Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read... * Nuke most topics under computation and many under reference (Dan) * Link all the golden gate materials (and work on general GGA materials) * PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ) * Migrate LTEE stuff to LTEE website (Jeff) * Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler) * <del>[[SoftwareList][Computation tool list]] -- Add pLannotate and OSTIR (Cameron)</del> * <del>[[LabPersonnel][Team]] – add start dates, be sure no former lab members were not transferred there.</del> * <del>Update research interests page (Jeff)</del> * <del>[[ProtocolsLearnBiocomputing][Anaconda should not be recommended]]</del> * Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s). * Add information about Lactobacillus strains in bees. * Troubleshooting minipreps, handling isolation of large plasmids
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Topic revision: r42 - 2025-05-20 - IraZibbu
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