How to Create a Protocol

Tips for Design

You should generally organize a protocol to have sections that are relevant from this list:

• Supplies (materials, strains, primers)
• Step-by-step instructions for each step
• Expected results (quantitative information, graphics, images)
• Common problems and troubleshooting
• Spreadsheets for calculations

Include instructions that reference specific reagents (strains, primers, etc.) for performing positive and negative control reactions. It is very important and can save a great deal of time if you run these reactions to diagnose bad reagents or a general problem with carrying out the protocol versus a problem with applying it in a specific case.

These protocols are public, so don't post passwords, protected information, or copyrighted material. Use the internal Barrick Lab web pages for that.

How to Add Images

1. Click the "attach" link at the bottom of your protocol page
2. Choose your image file:
   • Avoid spaces in the file name. Use underscores instead.
   • Under "Properties" check the box called "Create a link to the attached file."
3. After you click "Upload file" your image will appear at the bottom of the page after a bullet point.
4. Now edit the page and move the tag to where you want it displayed in the page.

Tips for Editing the Wiki

• Edit other protocol pages to see how to do things in Wiki syntax and make the formatting or your protocol consistent with others.
• We will eventually add some specific tags to make formatting references and contributors easier...