Contamination check. Set up plates with a checkerboard pattern of Ara+ and Ara- marked cells. On odd-numbered plates an Ara- is in well A1. On even-numbered plates an Ara+ strain is in well A1.

Daily, transfer 3.5 µl into 900 µl of fresh DM0 + 0.01% carbon compound.

At regular intervals, take a plate after it has

Plate 1

Strains 606/607. 8 Replicates, one per column.

-- Main.JeffreyBarrick - 24 Sep 2007


This topic: Lab > WebHome > ProceduresChangingEnvironmentLongTerm
Topic revision: r1 - 2007-09-24 - JeffreyBarrick