---+++ Contamination check. Set up plates with a checkerboard pattern of Ara+ and Ara- marked cells. On odd-numbered plates an Ara- is in well A1. On even-numbered plates an Ara+ strain is in well A1. Daily, transfer 3.5 µl into 900 µl of fresh DM0 + 0.01% carbon compound. At regular intervals, take a plate after it has ---++++ Plate 1 Red Plate. Strains 606/607. 8 Replicates, one per column. | 1 | 1 | D-Glucose | | 2 | 2 | Glycerol | | 3 | 3 | β-Lactose | | 4 | 4 | D-Fructose | | 5 | 5 | D-Ribose | | 6 | 6 | D-Galactose | | 7 | 7 | L-Rhamnose | | 8 | 8 | Melibiose | | 9 | 9 | D-Gluconic Acid | | 10 | 10 | D-Sorbitol | | 11 | 11 | Acetate | | 12 | 12 | L-Malic Acid | ---++++ Plate 2 White Plate. Strains 606/607. 8 Replicates, one per column. | 1 | 13 | Pyruvic Acid | | 2 | 14 | DL-Lactic Acid | | 3 | 17 | Thymidine | | 4 | 18 | Inosine | | 5 | 19 | D-Trehalose| | 6 | 20 | D-Serine | | 7 | 21 | D-Gluconic Acid | | 8 | 22 | L-Alanine | | 9 | 23 | D-Glucosamine | | 10 | 37 | Glucuronamide | | 11 | 71 | D-Arabinose | | 12 | 72 | L-Proline | ---++++ Plate 3 Red Plate. Strains 606/607. 8 Replicates, one per column. | | Treatment | Days | | 1 | L-Proline, Thymidine, Acetate, D-Glucose | 1 | | 2 | L-Proline, Thymidine, Acetate, D-Glucose | 2 | | 3 | L-Proline, Thymidine, Acetate, D-Glucose | 4 | | 4 | L-Proline, Thymidine, Acetate, D-Glucose | 8 | | 5 | L-Proline, Thymidine, Acetate, D-Glucose | 16 | | 6 | L-Proline, Thymidine, Acetate, D-Glucose | 32 | | 7 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 1 | | 8 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 2 | | 9 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 4 | | 10 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 8 | | 11 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 16 | | 12 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 32 | ---++++ Plate 4 White Plate. Strains 606/607. 8 Replicates, one per column. | | Treatment | Days | | 1 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 1 | | 2 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 2 | | 3 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 4 | | 4 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 8 | | 5 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 16 | | 6 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 32 | | 7 | Acetate, D-Ribose, Glycerol, D-Glucose | 1 | | 8 | Acetate, D-Ribose, Glycerol, D-Glucose | 2 | | 9 | Acetate, D-Ribose, Glycerol, D-Glucose | 4 | | 10 | Acetate, D-Ribose, Glycerol, D-Glucose | 8 | | 11 | Acetate, D-Ribose, Glycerol, D-Glucose | 16 | | 12 | Acetate, D-Ribose, Glycerol, D-Glucose | 32 | ---++++ Plate 5 Red Plate. Strains 606/607. 8 Replicates, one per column. | | Treatment | Days | | 1 | Random 1 | 1 | | 2 | Random 2 | 1 | | 3 | Random 3 | 1 | | 4 | Random 4 | 1 | | 5 | Random 5 | 1 | | 6 | Random 6 | 1 | | 7 | Random 7 | 1 | | 8 | Random 8 | 1 | | 9 | Random 9 | 1 | | 10 | Random 10 | 1 | | 11 | Random 11 | 1 | | 12 | Random 12 | 1 | -- Main.JeffreyBarrick - 24 Sep 2007
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Topic revision: r2 - 2007-09-24 - JeffreyBarrick