Day 1: Plating the Mixed Population
- Find microsatellite-containing strains in the -80°C freezer. They will be labeled with the appropriate microsatellite sequence located in the rhamnose operon (eg. "(CA)12"). See below for the list of microsatellites.
Day 2: Picking Colonies
Day 3: Passaging Colonies
| Number |
Sequence |
Position |
Fluorescent Tag? |
| 1 |
(CA)6 |
rhaT-rhaD |
|
| 2 |
(CA)12 |
rhaT-rhaD |
|
| 3 |
(TA)12 |
rhaT-rhaD |
|
| 4 |
(A)24 |
rhaT-rhaD |
|
| 5 |
(AGA)8 |
rhaT-rhaD |
|
| 6 |
(GGA)8 |
rhaT-rhaD |
|
| 7 |
(G)9 |
rhaT-rhaD |
|
| 8 |
(G)12 |
rhaT-rhaD |
|
-- Main.LindseyWolf - 18 May 2011
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Topic revision: r1 - 2011-05-18 - LindseyWolf