Day 1: Plating the Mixed Population
- Find microsatellite-containing strains in the -80°C freezer. They will be labeled with the appropriate microsatellite sequence located in the rhamnose operon (eg. "(CA)12"). See below for the list of microsatellites.
Day 2: Picking Colonies
Day 3: Passaging Colonies
| Number | Sequence | Position | Fluorescent Tag? |
|---|---|---|---|
| 1 | (CA)6 | rhaT-rhaD | |
| 2 | (CA)12 | rhaT-rhaD | |
| 3 | (TA)12 | rhaT-rhaD | |
| 4 | (A)24 | rhaT-rhaD | |
| 5 | (AGA)8 | rhaT-rhaD | |
| 6 | (GGA)8 | rhaT-rhaD | |
| 7 | (G)9 | rhaT-rhaD | |
| 8 | (G)12 | rhaT-rhaD |
This topic: Lab > WebHome > ProtocolList > ProcedureEvolvabilityMutationAccumulation
Topic revision: r1 - 2011-05-18 - LindseyWolf
