This page serves as the main repository for everything Golden Gate Assembly-related. This page links to a number of protocol pages that will help you design DNA sequences for Golden Gate Assembly, learn about the various different Golden Gate Assembly techniques and when we employ each one, and conduct the different types of Golden Gate Assembly reactions.

Golden Gate Assembly (GGA) was first described in Engler C, Kandzia R, Marillonnet S (2008) and Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) as an efficient way to quickly assembly multiple DNA sequences, or parts, into a single plasmid.

List of Golden Gate Assembly subpages

• Yeast Toolkit (YTK) - Derived from Lee, Dueber 2015
• Broad Host range Toolkit (BTK) - Derived from Leonard, Barrick 2018
• Designing a new DNA part sequence
• Creating a part plasmid using BsmBI
• Creating a cassette plasmid using BsaI

Work in Progress. As of 12/14/2017, none of the above links are guaranteed to work or be accurate. This is a work in progress reorganization of the Barrick lab Golden Gate Assembly Protocols, including those used by the Microbe Hackers undergraduate research lab and UT Austin iGEM team.

-Dr. Mishler