<<[[qPCR][Return to qPCR page]] ---+ Experimental qPCR Plate Setup and Analysis _Goals_ * Test hypothesis * Typical plate setup with 2 reference genes and a target gene: <table width="355" cellspacing="0" style="width: 355pt; margin-left: 4.65pt; border-collapse: collapse" cellpadding="0" border="0"> <tbody><tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> </td> <td width="40" valign="bottom" style="width: 40pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *1* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *2* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *3* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *4* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *5* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *6* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *7* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *8* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *9* </p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *A* </td> <td width="47" valign="bottom" style="width: 46.75pt; border: 1pt solid windowtext; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"><p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *B* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *C* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *D* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *E* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *F* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *G* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *H* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *I* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> *J* </td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> </tr> </tbody></table> _Conditions_ * If you want to be extra safe you should also include a negative control (usually DIW) for each primer pair. * The cDNA dilution used for the experimental samples will be those determined from the cDNA Concentration qPCR, and you should use at least your best 2 reference genes from the Reference Gene qPCR. _What you are looking for_ * Technical replicates with a standard deviation below 0.2. * Outliers; if you have an SD for a technical triplicate higher than 0.2, and there is obviously an outlier, remove it. An example is if you have values of 23.34, 23.35 and 30.22, and the 30.22 amplification trace is clearly poor. If the SD is higher than 0.2 and all three measurements are spread, you must keep all three. <<[[qPCR][Return to qPCR page]]
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Topic revision: r2 - 2020-04-09 - JuliePerreau