---++++ Plate 1: Single Carbon Sources, Glucose and Glycerol Timescale | 1 | 1 | D-Glucose | | 2 | 2 | Glycerol | | 3 | 3 | D-Ribose | | 4 | 4 | Pyruvate | | 5 | 5 | L-Malate | | 6 | 6 | Acetate | | 7 | 2/1 | Glycerol / D-Glucose, 1 transfer | | 8 | 2/1 | Glycerol / D-Glucose, 2 transfers | | 9 | 2/1 | Glycerol / D-Glucose, 4 transfers | | 10 | 2/1 | Glycerol / D-Glucose, 8 transfers | | 11 | 2/1 | Glycerol / D-Glucose, 16 transfers | | 12 | 2/1 | Glycerol / D-Glucose, 32 transfers | ---++++ Plate 2: Glucose and D-Ribose or Pyruvate Timescales | 1 | 3/1 | D-Ribose / D-Glucose, 1 transfer | | 2 | 3/1 | D-Ribose / D-Glucose, 2 transfers | | 3 | 3/1 | D-Ribose / D-Glucose, 4 transfers | | 4 | 3/1 | D-Ribose / D-Glucose, 8 transfers | | 5 | 3/1 | D-Ribose / D-Glucose, 16 transfers | | 6 | 3/1 | D-Ribose / D-Glucose, 32 transfers | | 1 | 4/1 | Pyruvate / D-Glucose, 1 transfer | | 2 | 4/1 | Pyruvate / D-Glucose, 2 transfers | | 3 | 4/1 | Pyruvate / D-Glucose, 4 transfers | | 4 | 4/1 | Pyruvate / D-Glucose, 8 transfers | | 5 | 4/1 | Pyruvate / D-Glucose, 16 transfers | | 6 | 4/1 | Pyruvate / D-Glucose, 32 transfers | ---++++ Plate 3: D-Glucose and L-Malate or Acetate Timescales | 7 | 5/1 | L-Malate / D-Glucose, 1 transfer | | 8 | 5/1 | L-Malate / D-Glucose, 2 transfers | | 9 | 5/1 | L-Malate / D-Glucose, 4 transfers | | 10 | 5/1 | L-Malate / D-Glucose, 8 transfers | | 11 | 5/1 | L-Malate / D-Glucose, 16 transfers | | 12 | 5/1 | L-Malate / D-Glucose, 32 transfers | | 7 | 6/1 | Acetate / D-Glucose, 1 transfer | | 8 | 6/1 | Acetate / D-Glucose, 2 transfers | | 9 | 6/1 | Acetate/ D-Glucose, 4 transfers | | 10 | 6/1 | Acetate/ D-Glucose, 8 transfers | | 11 | 6/1 | Acetate/ D-Glucose, 16 transfers | | 12 | 6/1 | Acetate/ D-Glucose, 32 transfers | ---++++ Plate 4: Other carbon compound pairs | 7 | 3/2 | D-Ribose / Glycerol, 8 transfers | | 8 | 4/2 | Pyruvate/ Glycerol, 8 transfers | | 9 | 5/2 | L-Malate / Glycerol, 8 transfers | | 10 | 6/2 | Acetate/ Glycerol, 8 transfers | | 11 | 4/3 | Pyruvate / D-Ribose, 8 transfers | | 12 | 5/3 | Acetate / D-Ribose, 8 transfers | | 7 | 6/3 | Acetate / D-Ribose, 8 transfer | | 8 | 5/4 | Acetate / Pyruvate, 8 transfers | | 9 | 6/4 | Acetate/ Pyruvate, 8 transfers | | 10 | 6/5 | Acetate/ L-Malate, 8 transfers | | 11 | | Blanks | | 12 | | | ---++++ Plate 1: 12 single carbon sources General design considerations: 1 Begin growth in substrate that supports lower cell density. Red Plate. Strains 606/607. 8 Replicates, one per column. | 1 | 1 | D-Glucose | 1 | | 2 | 2 | D-Mannose | 1? | | 3 | 3 | D-Galactose | 1 | | 4 | 4 | Glycerol | 1 | | 5 | 5 | D-Ribose | 2 | | 6 | 6 | β-Lactose | 2 | | 7 | 7 | L-Rhamnose | 4 | | 8 | 8 | L-Alanine | 4 | | 9 | 9 | Pyruvate | 4 | | 10 | 10 | D-Sorbitol | 5 | | 11 | 11 | Acetate | 8 | | 12 | 12 | L-Malic Acid | 8 | ---++++ Plate 2: glucose / lactose scan + 5 carbon source pairs | 1 | 6/1 | β-Lactose / D-Glucose, 1 transfer | | 2 | 6/1 | β-Lactose / D-Glucose, 2 transfers | | 3 | 6/1 | β-Lactose / D-Glucose, 4 transfers | | 4 | 6/1 | β-Lactose / D-Glucose, 8 transfers | | 5 | 6/1 | β-Lactose / D-Glucose, 16 transfers | | 6 | 6/1 | β-Lactose / D-Glucose, 32 transfers | | 7 | 6/1 | β-Lactose / D-Glucose, 64 transfers | | 8 | 2/1 | D-Mannose / D-Glucose, 8 transfers | | 9 | 3/1 | D-Galactose / D-Glucose | | 10 | 4/1 | Glycerol / D-Glucose | | 11 | 5/1 | D-Ribose / D-Glucose | | 12 | 7/1 | L-Rhamnose / D-Glucose | ---++++ Plate 3: glucose / pyruvate scan + 5 carbon source pairs | 1 | 9/1 | Pyruvate / D-Glucose, 1 transfer | | 2 | 9/1 | Pyruvate / D-Glucose, 2 transfers | | 3 | 9/1 | Pyruvate / D-Glucose, 4 transfers | | 4 | 9/1 | Pyruvate / D-Glucose, 8 transfers | | 5 | 9/1 | Pyruvate / D-Glucose, 16 transfers | | 6 | 9/1 | Pyruvate / D-Glucose, 32 transfers | | 7 | 9/1 | Pyruvate / D-Glucose, 64 transfers | | 8 | 8/1 | L-Alanine / D-Glucose, 8 transfers | | 9 | 10/1 | D-Sorbitol / D-Glucose, 8 transfers | | 10 | 11/1 | Acetate / D-Glucose, 8 transfers | | 11 | | | | 12 | | | 2-4 2-5 2-6 2-7 2-10 2-11 3-5 3-6 3-7 3-9 3-10 4-7 4-8 4-9 4-10 5-6 5-10 5-11 5-12 6-8 6-12 7-8 7-9 7-10 7-12 8-11 8-12 9-10 10-11 10-12 11-12 ---++++ Plate 4: glucose / L-malate scan + 5 carbon source pairs | 1 | 12/1 | D-Glucose / L-Malate, 1 transfer | | 2 | 12/1 | D-Glucose / L-Malate, 2 transfers | | 3 | 12/1 | D-Glucose / L-Malate, 4 transfers | | 4 | 12/1 | D-Glucose / L-Malate, 8 transfers | | 5 | 12/1 | D-Glucose / L-Malate, 16 transfers | | 6 | 12/1 | D-Glucose / L-Malate, 32 transfers | | 7 | 12/1 | D-Glucose / L-Malate, 64 transfers | ---++++ Plate 5: 12 carbon source pairs ---++++ Plate 6: 12 carbon source pairs ---+++ First Generation Plates ---++++ Plate 1 Red Plate. Strains 606/607. 8 Replicates, one per column. | 1 | 1 | D-Glucose | | 2 | 2 | Glycerol | | 3 | 3 | β-Lactose | | 4 | 4 | D-Fructose | | 5 | 5 | D-Ribose | | 6 | 6 | D-Galactose | | 7 | 7 | L-Rhamnose | | 8 | 8 | Melibiose | | 9 | 9 | D-Gluconic Acid | | 10 | 10 | D-Sorbitol | | 11 | 11 | Acetate | | 12 | 12 | L-Malic Acid | ---++++ Plate 2 White Plate. Strains 606/607. 8 Replicates, one per column. | 1 | 13 | Pyruvic Acid | | 2 | 14 | DL-Lactic Acid | | 3 | 17 | Thymidine | | 4 | 18 | Inosine | | 5 | 19 | D-Trehalose| | 6 | 20 | D-Serine | | 7 | 21 | D-Glucuronic Acid | | 8 | 22 | L-Alanine | | 9 | 23 | D-Glucosamine | | 10 | 37 | Glucuronamide | | 11 | 71 | D-Arabinose | | 12 | 72 | L-Proline | ---++++ Plate 3 Red Plate. Strains 606/607. 8 Replicates, one per column. | | Treatment | Transfers | | 1 | L-Proline, Thymidine, Acetate, D-Glucose | 1 | | 2 | L-Proline, Thymidine, Acetate, D-Glucose | 2 | | 3 | L-Proline, Thymidine, Acetate, D-Glucose | 4 | | 4 | L-Proline, Thymidine, Acetate, D-Glucose | 8 | | 5 | L-Proline, Thymidine, Acetate, D-Glucose | 16 | | 6 | L-Proline, Thymidine, Acetate, D-Glucose | 32 | | 7 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 1 | | 8 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 2 | | 9 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 4 | | 10 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 8 | | 11 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 16 | | 12 | Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose | 32 | ---++++ Plate 4 White Plate. Strains 606/607. 8 Replicates, one per column. | | Treatment | Transfers | | 1 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 1 | | 2 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 2 | | 3 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 4 | | 4 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 8 | | 5 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 16 | | 6 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 32 | | 7 | Acetate, D-Ribose, Glycerol, D-Glucose | 1 | | 8 | Acetate, D-Ribose, Glycerol, D-Glucose | 2 | | 9 | Acetate, D-Ribose, Glycerol, D-Glucose | 4 | | 10 | Acetate, D-Ribose, Glycerol, D-Glucose | 8 | | 11 | Acetate, D-Ribose, Glycerol, D-Glucose | 16 | | 12 | Acetate, D-Ribose, Glycerol, D-Glucose | 32 |
This topic: Lab
>
WebHome
>
ProtocolList
>
EvolvabilityLongTermPlates
Topic revision: r6 - 2007-11-14 - JeffreyBarrick