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TWiki's Lab web
  https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ResearchInterests https://barricklab.org/twiki/bin/view/Lab/ResearchInterests Barrick Lab :: Research Listen to an introduction to our synthetic biology research:Evolution and Engineering Bee Guts (EBRC in Translation Podcast) Watch a public... (last changed by AlexaMorton) 2025-06-12T19:17:25Z AlexaMorton ProtocolsAntibioticStockSolutions https://barricklab.org/twiki/bin/view/Lab/ProtocolsAntibioticStockSolutions Media amendments Preparation: Generally, prepare 30 50 mL of solution in a 50 mL conical tube. Then, filter sterilize solutions by pushing them through a 50 mL syringe... (last changed by AlexaMorton) 2025-06-11T19:01:08Z AlexaMorton ProtocolsAcinetobacterOverlapPCRTransformation https://barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterOverlapPCRTransformation Acinetobacter baylyi ADP1 Genome Manipulations by Overlap Extension PCR Overlap PCR Construct Generation The following is a standard procedure designing and constructing... (last changed by CameronRoots) 2025-03-20T21:37:57Z CameronRoots ProtocolsRecipesCzapekBroth https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesCzapekBroth Czapek Broth (CB) Combine in a flask: 500 mL 1 L Component 1.5 g 3 g sodium nitrate 0.5 g 1 g potassium phosphate dibasic 0.25 g... (last changed by MeghnaVergis) 2024-10-16T02:49:56Z MeghnaVergis ProtocolsConjugation https://barricklab.org/twiki/bin/view/Lab/ProtocolsConjugation General Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol... (last changed by KadenaCope) 2024-09-25T18:17:22Z KadenaCope ProtocolsCFUCounts https://barricklab.org/twiki/bin/view/Lab/ProtocolsCFUCounts CFU Counts This is an outline for a general protocol to assess the number of colony forming units in a sample using spot plating. This method cuts down on the number... (last changed by KateElston) 2022-08-17T17:21:51Z KateElston RefGeneqPCR https://barricklab.org/twiki/bin/view/Lab/RefGeneqPCR C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Template Material Use cDNA dilution determined in cDNA Concentration qPCR Note: Once you... (last changed by KateElston) 2022-07-19T22:56:58Z KateElston ProtocolsP1Transduction https://barricklab.org/twiki/bin/view/Lab/ProtocolsP1Transduction P1 Transduction in Escherichia coli This protocol is adapted from Thomason, L. C., Costantino, N. and Court, D. L. 2007. E. coli Genome Manipulation by P1 Transduction... (last changed by JeffreyBarrick) 2021-07-15T16:15:07Z JeffreyBarrick

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