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TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsRecipesSOB https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSOB SOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by AlexaMorton) 2025-07-03T16:59:00Z AlexaMorton PastLabPersonnel https://barricklab.org/twiki/bin/view/Lab/PastLabPersonnel Past Barrick Lab Members Cameron Roots Jeffrey Chuong Kadena Cope Sophia Lin Olivia Soto Jack Dwenger Elizabeth Manriquez Dan Deathrage Victor Li Matt McGuffie Laurel... (last changed by AlexaMorton) 2025-06-12T20:17:03Z AlexaMorton ProtocolsGettingStarted https://barricklab.org/twiki/bin/view/Lab/ProtocolsGettingStarted Getting Started with Lab Techniques General molecular lab techniques Engineering: A Primer to Get You Started General microbiology lab techniques... (last changed by LucioNavarro) 2025-03-20T21:47:08Z LucioNavarro ProtocolsWorkingWithSerratiaSymbiotica https://barricklab.org/twiki/bin/view/Lab/ProtocolsWorkingWithSerratiaSymbiotica Working with Serratia symbiotica Serratia symbiotica is a secondary endosymbiont of the black bean aphid ( Aphis fabae ) gut microbiota that can be cultivated... (last changed by AnthonyVandieren) 2025-03-20T21:24:12Z AnthonyVandieren ProtocolsRecipesLysogenyBrothAgar https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesLysogenyBrothAgar LB: Lysogeny Broth / Luria Bertani (Miller) Agar Add dH2O to full volume in an appropriately sized flask. Combine the following, making sure each component fully dissolves... (last changed by TylerDeJong) 2025-02-19T19:00:49Z TylerDeJong ProtocolsComputingEnvironmentSetup https://barricklab.org/twiki/bin/view/Lab/ProtocolsComputingEnvironmentSetup Computing Environment Setup for Bioinformatics and Computational Biology So, you want to harness the immense power of bioinformatics and computational biology for... (last changed by JeffreyBarrick) 2024-07-09T22:37:14Z JeffreyBarrick ProtocolsReagentRecipes https://barricklab.org/twiki/bin/view/Lab/ProtocolsReagentRecipes Reagent and Buffer Recipes General calculation resources Mass Molarity Calculator Solution Dilution Calculator Gel Electrophoresis 50 TAE (Tris Acetate... (last changed by IsaacGifford) 2023-09-20T16:31:05Z IsaacGifford ProtocolsGoldenGateTroubleshooting https://barricklab.org/twiki/bin/view/Lab/ProtocolsGoldenGateTroubleshooting Back to Golden Gate Protocols Troubleshooting Golden Gate Assemblies Tips Screen Inserts for internal BsaI/BsmBI sites! Reactions with single off target sites... (last changed by JeffreyBarrick) 2023-06-14T17:43:25Z JeffreyBarrick
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TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProceduresLongTermCompetitions https://barricklab.org/twiki/bin/view/Lab/ProceduresLongTermCompetitions Co culture Competition Assays The instructions below include the basic protocol. Be sure to check whether there are variations needed for your specific samples! For... (last changed by JeffreyBarrick) 2025-06-05T11:20:32Z JeffreyBarrick ProtocolsRecipesDavisMingioli https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli DM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by AlexaMorton) 2025-05-20T18:22:19Z AlexaMorton ProtocolUpdatesNeeded https://barricklab.org/twiki/bin/view/Lab/ProtocolUpdatesNeeded Protocol Updates Needed this page on DNA sequencing needs to be updated and be made more for the general public than to the lab General guide for sorting... (last changed by IraZibbu) 2025-05-20T14:41:14Z IraZibbu ProtocolsUnixCommandsQuickReference https://barricklab.org/twiki/bin/view/Lab/ProtocolsUnixCommandsQuickReference Unix Commands Quick Reference Useful commands and flags that we get tired of looking up... Alphabetical Reference cat yourfile.txt Prints the contents of the given... (last changed by JeffreyBarrick) 2024-07-09T22:32:58Z JeffreyBarrick Strain_side_notes https://barricklab.org/twiki/bin/view/Lab/Strain_side_notes Commonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points... (last changed by TylerDeJong) 2024-07-09T21:14:39Z TylerDeJong BioPythonOnboard https://barricklab.org/twiki/bin/view/Lab/BioPythonOnboard (last changed by JeffreyBarrick) 2023-09-20T15:41:36Z JeffreyBarrick ProtocolsBTKAssembleSingleTUPlasmid https://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid Back to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by KateElston) 2021-11-03T20:57:10Z KateElston ProtocolsGibsonCloning https://barricklab.org/twiki/bin/view/Lab/ProtocolsGibsonCloning Gibson Assembly Background and Design Gibson Cloning is a technique of DNA construct assembly that allows one to join multiple linear segments into either one large... (last changed by CameronRoots) 2021-10-22T16:09:24Z CameronRoots
 

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