Lab WebRss (1 vs. 2)

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<<O>> Difference Topic WebRss (2 - 2004-08-16 - PeterThoeny)

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+  TWiki's Lab web
  https://barricklab.org/twiki/bin/view/Lab
  The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World.

  en-us
  Copyright 2025 by contributing authors
  TWiki Administrator [admin@barricklab.org]
  The contributing authors of TWiki
  TWiki
  
    
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
    
  


  Home - this site is powered by TWiki(R).Lab
  https://barricklab.org/twiki/bin/view/Lab
  /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif


  WebTopMenu
  https://barricklab.org/twiki/bin/view/Lab/WebTopMenu
  Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick)
  2025-09-05T18:23:35Z
  
    
      JeffreyBarrick
    
  


  WebHome
  https://barricklab.org/twiki/bin/view/Lab/WebHome
  .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)
  2025-09-01T17:23:56Z
  
    
      JeffreyBarrick
    
  


  FrontPageImages
  https://barricklab.org/twiki/bin/view/Lab/FrontPageImages
  Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick)
  2025-09-01T11:41:29Z
  
    
      JeffreyBarrick
    
  


  PublicationList
  https://barricklab.org/twiki/bin/view/Lab/PublicationList
  Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)
  2025-08-31T14:27:55Z
  
    
      JeffreyBarrick
    
  


  TopMenuSkinTopBar
  https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar
  Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick)
  2025-08-29T11:39:17Z
  
    
      JeffreyBarrick
    
  


  LabPersonnel
  https://barricklab.org/twiki/bin/view/Lab/LabPersonnel
  Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)
  2025-08-29T11:15:46Z
  
    
      JeffreyBarrick
    
  


  WebTopBar
  https://barricklab.org/twiki/bin/view/Lab/WebTopBar
   (last changed by JeffreyBarrick)
  2025-08-29T01:24:36Z
  
    
      JeffreyBarrick
    
  


  ContactInformation
  https://barricklab.org/twiki/bin/view/Lab/ContactInformation
  Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick)
  2025-08-15T16:29:20Z
  
    
      JeffreyBarrick
    
  


  GoldenGateAssemblyProtocolsMainPage
  https://barricklab.org/twiki/bin/view/Lab/GoldenGateAssemblyProtocolsMainPage
  Golden Gate Assembly This page serves as the main repository for everything Golden Gate Assembly related. This page links to a number of protocol pages that will... (last changed by AlexaMorton)
  2025-06-17T14:32:36Z
  
    
      AlexaMorton
    
  


  GlasswareCleaning
  https://barricklab.org/twiki/bin/view/Lab/GlasswareCleaning
  How to clean glassware LTEE flasks and lids LTEE glassware... (last changed by AlexaMorton)
  2025-06-12T19:13:08Z
  
    
      AlexaMorton
    
  


  ProtocolsRetired
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsRetired
  Retired Protocols Designing a new part Designing a new part for use in the BTK Bee Microbiome Toolkit (BTK) Golden Gate part kit for work in non model bacterial... (last changed by JeffreyBarrick)
  2025-02-10T19:14:28Z
  
    
      JeffreyBarrick
    
  


  BroadHostRangeToolkit
  https://barricklab.org/twiki/bin/view/Lab/BroadHostRangeToolkit
  Bee Microbiome Toolkit The Bee Microbiome Toolkit (BTK) is a Golden Gate compatible toolkit of genetic parts designed for working with newly isolated, non model bacteria... (last changed by CameronRoots)
  2024-12-13T17:34:59Z
  
    
      CameronRoots
    
  


  BioPythonOnboard
  https://barricklab.org/twiki/bin/view/Lab/BioPythonOnboard
   (last changed by JeffreyBarrick)
  2023-09-20T15:41:36Z
  
    
      JeffreyBarrick
    
  


  ExperimentalqPCR
  https://barricklab.org/twiki/bin/view/Lab/ExperimentalqPCR
  C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Conditions If you want to be extra safe you should also include a negative control (usually... (last changed by KateElston)
  2022-07-19T23:00:43Z
  
    
      KateElston
    
  


  ProtocolsRecipesYPDmedium
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesYPDmedium
  YPD Medium General media used for culturing Yeast. Adapted from spring harbor In 1 L bottle: 1L Component 20g Peptone 10 g Yeast Extract... (last changed by JeffreyBarrick)
  2022-06-01T18:44:45Z
  
    
      JeffreyBarrick
    
  


  ProtocolsGibsonCloning
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsGibsonCloning
  Gibson Assembly Background and Design Gibson Cloning is a technique of DNA construct assembly that allows one to join multiple linear segments into either one large... (last changed by CameronRoots)
  2021-10-22T16:09:24Z
  
    
      CameronRoots
->
>
+  TWiki's Lab web
  https://barricklab.org/twiki/bin/view/Lab
  The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World.

  en-us
  Copyright 2025 by contributing authors
  TWiki Administrator [admin@barricklab.org]
  The contributing authors of TWiki
  TWiki
  
    
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
      
    
  


  Home - this site is powered by TWiki(R).Lab
  https://barricklab.org/twiki/bin/view/Lab
  /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif


  WebTopMenu
  https://barricklab.org/twiki/bin/view/Lab/WebTopMenu
  Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick)
  2025-09-05T18:23:35Z
  
    
      JeffreyBarrick
    
  


  WebHome
  https://barricklab.org/twiki/bin/view/Lab/WebHome
  .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)
  2025-09-01T17:23:56Z
  
    
      JeffreyBarrick
    
  


  FrontPageImages
  https://barricklab.org/twiki/bin/view/Lab/FrontPageImages
  Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick)
  2025-09-01T11:41:29Z
  
    
      JeffreyBarrick
    
  


  PublicationList
  https://barricklab.org/twiki/bin/view/Lab/PublicationList
  Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)
  2025-08-31T14:27:55Z
  
    
      JeffreyBarrick
    
  


  TopMenuSkinTopBar
  https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar
  Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick)
  2025-08-29T11:39:17Z
  
    
      JeffreyBarrick
    
  


  LabPersonnel
  https://barricklab.org/twiki/bin/view/Lab/LabPersonnel
  Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)
  2025-08-29T11:15:46Z
  
    
      JeffreyBarrick
    
  


  WebTopBar
  https://barricklab.org/twiki/bin/view/Lab/WebTopBar
   (last changed by JeffreyBarrick)
  2025-08-29T01:24:36Z
  
    
      JeffreyBarrick
    
  


  ContactInformation
  https://barricklab.org/twiki/bin/view/Lab/ContactInformation
  Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick)
  2025-08-15T16:29:20Z
  
    
      JeffreyBarrick
    
  


  ProtocolsAntibioticStockSolutions
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsAntibioticStockSolutions
  Media amendments Preparation: Generally, prepare 30 50 mL of solution in a 50 mL conical tube. Then, filter sterilize solutions by pushing them through a 50 mL syringe... (last changed by AlexaMorton)
  2025-06-11T19:01:08Z
  
    
      AlexaMorton
    
  


  ListofBioinformatics
  https://barricklab.org/twiki/bin/view/Lab/ListofBioinformatics
  Bioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu)
  2025-04-17T21:59:52Z
  
    
      IraZibbu
    
  


  HMWDNA
  https://barricklab.org/twiki/bin/view/Lab/HMWDNA
  Handling High Molecular Weight DNA Introduction Long read sequencing, such as Oxford Nanopore or PacBio sequencing, is becoming increasingly important for genome... (last changed by IsaacGifford)
  2025-04-08T15:40:58Z
  
    
      IsaacGifford
    
  


  ProtocolsRecipesTetrazoliumSugar
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesTetrazoliumSugar
  TA: Tetrazolium Sugar (TA, TM, TL, ...) Combine in a 1 L flask: 500 mL Component 5 g Tryptone 500 mg Yeast Extract 2.5 g NaCl 8 g... (last changed by AlexaMorton)
  2025-01-09T20:04:48Z
  
    
      AlexaMorton
    
  


  ProtocolsBTKMakeANewPartPlasmid
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKMakeANewPartPlasmid
  Back to Golden Gate Protocols Part Plasmid Assembly Once you have designed your part and either amplified with PCR or ordered the desired gBlock (as described here... (last changed by JeffreyBarrick)
  2023-06-14T17:33:34Z
  
    
      JeffreyBarrick
    
  


  ProcedureDNASequencing
  https://barricklab.org/twiki/bin/view/Lab/ProcedureDNASequencing
  Sanger DNA Sequencing Go to the UT DNA Sequencing Facility website. This page explains the pricing for various orders and the methods by which samples should be prepared... (last changed by ZuberiAshraf)
  2023-02-27T18:32:51Z
  
    
      ZuberiAshraf
    
  


  FreezerFailure
  https://barricklab.org/twiki/bin/view/Lab/FreezerFailure
  Sensaphone Protocol The sensaphone is the alarm monitor connected to both of the 80C freezers in MBB. Below is an overview of the current settings and the expected... (last changed by DanielDeatherage)
  2023-01-11T20:06:04Z
  
    
      DanielDeatherage
    
  


  ProtocolsLeafhoppers
  https://barricklab.org/twiki/bin/view/Lab/ProtocolsLeafhoppers
  Leafhopper Care and Protocols Rearing and Caring for Leafhopper Species Leafhopper species are kept in BugDorms (BugDorm 4F3030 and 4F2222). We have the following... (last changed by SarahBialik)
  2021-11-03T20:09:36Z
  
    
      SarahBialik
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<<O>> Difference Topic WebRss (1 - 2003-01-30 - PeterThoeny)

TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsTaq https://barricklab.org/twiki/bin/view/Lab/ProtocolsTaq Which polymerase is right for me? Types of polymerase NEB polymerases may be purchased from the NEB freezer, which is located in the Biomedical Research Supply Core... (last changed by MeghnaVergis) 2025-03-20T21:27:28Z MeghnaVergis ProtocolsOrderingPrimers https://barricklab.org/twiki/bin/view/Lab/ProtocolsOrderingPrimers Ordering Primers 1 Go to ICMB`s IDT webpage ICMB`s IDT page 1 Click Set up new Account tab on bottom left. 1 Fill out all of your information, including... (last changed by JeffreyBarrick) 2022-09-26T21:55:23Z JeffreyBarrick RefGeneqPCR https://barricklab.org/twiki/bin/view/Lab/RefGeneqPCR C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Template Material Use cDNA dilution determined in cDNA Concentration qPCR Note: Once you... (last changed by KateElston) 2022-07-19T22:56:58Z KateElston CDNAConcentrationqPCR https://barricklab.org/twiki/bin/view/Lab/CDNAConcentrationqPCR (Numbers are cDNA dilution with `5` representing a 1:5 dilution and so on) Template Material The cDNA used for this is a pool of all your cDNA samples i.e.... (last changed by KateElston) 2022-07-19T22:55:06Z KateElston SupplementLongTermMutationRates https://barricklab.org/twiki/bin/view/Lab/SupplementLongTermMutationRates Supplementary Scripts and Data Files Mutation rate inferred from synonymous substitutions in a long term evolution experiment with Escherichia coli Wielgoss, et... (last changed by JeffreyBarrick) 2022-04-22T14:30:55Z JeffreyBarrick ProtocolsTestingTransformEff https://barricklab.org/twiki/bin/view/Lab/ProtocolsTestingTransformEff Checking Transformation Efficiency of Chemically Competent Cells Adapted from Molecular Cloning: A Laboratory Manual 3rd Ed. , Sambrook and Russell (2001) SUPPLIES... (last changed by JeffreyBarrick) 2021-07-05T20:39:06Z JeffreyBarrick ProtocolsAcinetobacterGenomeManipulation https://barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterGenomeManipulation Acinetobacter baylyi ADP1 Genome Manipulations Genome manipulations in Acinetobacter baylyi ADP1 can be performed without the need for exogenous recombinase expression... (last changed by IsaacGifford) 2020-12-03T22:17:10Z IsaacGifford ProtocolsBroccoliAptamerInVitroAssays https://barricklab.org/twiki/bin/view/Lab/ProtocolsBroccoliAptamerInVitroAssays Measuring transcription in vitro Using Broccoli and Spinach Fluorescent RNA Aptamers: Background / Usage: Broccoli and Spinach are two versions of an RNA aptamer... (last changed by ColinBrown) 2018-03-29T16:41:52Z ColinBrown

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