| |
S2
Used for Acinetobacter |
|
<
< |
| 1L |
5L |
Component |
| 1.5g |
7.5 g |
KH2PO4 |
| 25.5g |
127.5 g |
Na2HPO4 7H2O |
|
>
> | Recipe for 900mL
(Autoclave in three separate bottles - 300mL each) |
|
<
< |
|
| | |
|
<
< | After autoclaving, wait for containers to return to room temperature, then add the following sterile ingredients. |
>
> | Bottle 1 (Erlenmeyer flask: 300mL) |
|
>
> |
| 300mL |
Component |
| 1.35g |
KH2PO4 (monobasic) |
| 22.95g |
Na2HPO4 7H2O |
|
| | |
|
<
< |
| 1L |
Component |
| 1ml |
10% MgSO4 |
| 0.09ul |
1M CaCl2 |
|
>
> | Bottle 2 (Erlenmeyer flask: 300mL)
| 300mL |
Component |
| 0.09g |
MgSO4 |
|
|
<
< |
| 0.5ml |
0.1% FeSO4 7H2O |
| 5 ml |
90% Lactic Acid |
|
| | |
|
>
> | Bottle 3 (1L bottle: 300mL)
| 300mL |
Component |
| 1.8g |
NH4Cl |
| 81ul |
1M CaCl2 |
After autoclaving, let these reach room temperature and transfer all into one 1L bottle (Bottle 3), then, finally add the following sterile components:
| 0.45ml |
0.1% FeSO4 7H2O |
| 4.5 ml |
90% Lactic Acid |
|
| | The functional constraints are that Calcium Chloride cannot be autoclaved in the presence of sulfates, or it will precipitate as calcium sulfate. Ferrous sulfate when autoclaved will decompose to ferric sulfate, ferric oxide and sulfur dioxide. This means that either calcium chloride OR magnesium sulfate may be added before autoclaving, but not both.
| Final composition |
| 190.2 mM |
Sodium (Na+) |
| 11.0 mM |
Potassium (K+) |
| 37.8 mM |
Ammonium (NH4+) |
| 0.09 mM |
Calcium (Ca2+) |
| 0.83 mM |
Magnesium (Mg2+) |
| 0.033 mM |
Iron II (Fe2+) |
| 38.0 mM |
Chloride (Cl-) |
| 0.87 mM |
Sulfate (SO42-) |
| 106.1 mM |
Phosphate (PO43-) |
| 50.0 mM |
Lactic Acid |
|
|
<
< | -- Main.AurkoDasgupta - 03 Jun 2013 |
>
> | -- Main.GabrielSuarez - 05 Sep 2013 |
| | |
