| |
| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
M9 Minimal Media
|
|
<
< |
| 6 g |
Sodium phosphate, Na2HPO4 |
| 3 g |
Potassium phosphate, KH2PO4 |
| 0.5 g |
Sodium chloride, NaCl |
|
>
> |
| 6 g |
Sodium phosphate dibasic (anhydrous), Na2HPO4 |
| 3 g |
Potassium phosphate monobasic, KH2PO4 |
| 0.5 g |
Sodium chloride, NaCl |
|
| |
| 1 g |
Ammonium chloride, NH4Cl |
Add dH2O to 1 liter. Autoclave.
After media is autoclaved add the following sterile ingredients:
|
|
<
< |
| 1 ml |
1M Magnesium sulfate, MgSO4 |
| 1 ml |
0.1M Calcium chloride CaCl2 |
|
>
> |
| 1 ml |
1M Magnesium sulfate, MgSO4 |
| 1 ml |
0.1M Calcium chloride CaCl2 |
|
| |
| 10 ml |
10% (w/v) glucose (or other carbon source) |
| Final composition |
| 93.0 mM |
Sodium (Na+) |
| 22.1 mM |
Potassium (K+) |
| 18.7 mM |
Ammonium (NH4) |
| 1.0 mM |
Calcium (Ca2+) |
| 0.1 mM |
Magnesium (Mg2+) |
| 29.2 mM |
Chloride (Cl- |
| 0.1 mM |
Sulfate (SO42-) |
| 42.2 mM |
Phosphate (PO43-) |
|
