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| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
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|
<
< | M9 Minimal |
>
> | M9 Minimal Media |
| | |
|
<
< | Make 5× M9 salts: |
| |
|
|
<
< |
| 64 g |
Sodium phosphate (dibasic, 7 hydrate) Na2HPO4•H2O |
| 15 g |
Potassium phosphate (monobasic) KH2PO4 |
| 2.5 g |
Sodium chloride, NaCl |
| 5 g |
Ammonium chloride NH4Cl |
|
>
> |
| 6 g |
Sodium phosphate, Na2HPO4 |
| 3 g |
Potassium phosphate, KH2PO4 |
| 0.5 g |
Sodium chloride, NaCl |
| 1 g |
Ammonium chloride, NH4Cl |
|
| | Add dH2O to 1 liter. Autoclave. |
|
<
< | Combine the following ingredients (all pre-sterilized): |
>
> | After media is autoclaved add the following sterile ingredients: |
| |
|
|
<
< |
| 200 ml |
5× M9 salts |
| 2 ml |
1M Magnesium sulfate MgSO4 |
| 0.1 ml |
1 M Calcium chloride CaCl2 |
|
>
> |
| 1 ml |
1M Magnesium sulfate, MgSO4 |
| 1 ml |
0.1M Calcium chloride CaCl2 |
| 10 ml |
10% (w/v) glucose (or other carbon source) |
|
|
<
< |
| 40 ml |
10% (w/v) glucose (or other carbon source) |
Add sterile dH2O to 1 liter. Autoclave.
To make M9 plates add the items above to 16g of sterilized agar.
If making less than 1 liter use 8g of agar per 500ml of solution.
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