Difference:
ProtocolsRecipesDavisMingioli
(6 vs. 7)
Revision 7
2012-05-02 -
CraigBarnhart
META TOPICPARENT
name="ProtocolsMediaRecipes"
DM: Davis Minimal
1L
5L
Component
MW
7 g
35 g
Potassium Phosphate (dibasic, trihydrate) HK
2
PO
4
(H
2
O)
3
MW 228.17
2 g
10 g
Potassium Phosphate (monobasic) H
2
KPO
4
MW 136.07
1 g
5 g
Ammonium Sulfate (NH
4
)
2
SO
4
MW 132.08
0.5 g
2.5 g
Sodium Citrate (trisodium, dihydrate) Na
3
C
6
H
5
O
7
(H
2
O)
2
MW 294.10
Add dH
2
O to final volume and autoclave.
After autoclaving add the following stock solutions:
1L
5L
Component
1.0 ml
5 ml
10% Magnesium Sulfate MgSO
4
Changed:
<
<
1.0 ml
5 ml
0.2% Thiamin (vitamin B1) (filter sterilized)
>
>
1.0 ml
5 ml
0.2% Thiamine (vitamin B1) (filter sterilized)
Final nutrient concentrations:
67.4 mM
Phosphate (PO
4
3-
)
8.38 mM
Sulfate (SO
4
2-
)
15.1 mM
Ammonium (NH
4
)
5.1 mM
Sodium (Na
+
)
98.1 mM
Potassium (K
+
)
0.83 mM
Magnesium (MG
2+
)
1.7 mM
Citrate
139 µM
Glucose (in DM25)
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