| |
| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
DM: Davis-Mingioli
Growth medium used by the long-term E. coli evolution experiment.
| 0.5L |
1L |
4.4L |
5L |
Component |
MW |
| 2.67 g |
5.34 g |
23.32 g |
26.7 g |
Potassium Phosphate (dibasic) K2HPO4 |
MW 174.18 |
| 1 g |
2 g |
8 g |
10 g |
Potassium Phosphate (monobasic) KH2PO4 |
MW 136.07 |
| 0.5 g |
1 g |
4.4 g |
5 g |
Ammonium Sulfate (NH4)2 SO4 |
MW 132.08 |
| 0.25 g |
0.5 g |
2.2 g |
2.5 g |
Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 |
MW 294.10 |
Add dH2O to final volume and autoclave.
Note: 4.4 L of DM makes 8 bottles with 550 ml each.
Note: If using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L |
|
<
< | |
| | After autoclaving add the following stock solutions:
| 0.5L |
550mL |
1L |
5L |
Component |
|
|
<
< |
| 0.5 ml |
550 痞 |
1.0 ml |
5 ml |
10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock) |
|
>
> |
| 0.5 ml |
550 痞 |
1.0 ml |
5 ml |
10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock) |
|
| |
| 0.5 ml |
1000 痞 |
1.0 ml |
5 ml |
0.2% (w/v) Thiamine (vitamin B1) (filter sterilized) |
And supplement with a carbon source.
If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:
| 0.5L |
550mL |
1L |
5L |
DMX |
[glucose] (w/v) |
[glucose] (mg/L) |
[glucose] (M) |
| 125 痞 |
137.5 痞 |
250 痞 |
1.25 ml |
DM25 |
0.0025% |
25 mg/L |
139 然 |
|
|
<
< |
| 0.5 ml |
0.55 ml |
1 ml |
5 ml |
DM100 |
0.010% |
100 mg/L |
694 然 |
|
>
> |
| 0.5 ml |
0.55 ml |
1 ml |
5 ml |
DM100 |
0.010% |
100 mg/L |
694 然 |
|
| |
| 1.25 ml |
1.375 ml |
2.5 ml |
12.5 ml |
DM250 |
0.025% |
250 mg/L |
1.39 然 |
| 2.5 ml |
2.75 ml |
5 ml |
25 ml |
DM500 |
0.05% |
500 mg/L |
2.78 mM |
| 5 ml |
5.5 ml |
10 ml |
50 ml |
DM1000 |
0.1% |
1000 mg/L |
5.55 mM |
| 10 ml |
11 ml |
20 ml |
100 ml |
DM2000 |
0.2% |
2000 mg/L |
11.1 mM |
Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.
| Final composition |
| 5.1 mM |
Sodium (Na+) |
| 75.8 mM |
Potassium (K+) |
|
|
<
< |
|
>
> |
|
| |
| 0.83 mM |
Magnesium (Mg2+) |
| 8.41 mM |
Sulfate (SO42-) |
| 45.3 mM |
Phosphate (PO43-) |
| 1.70 mM |
Citrate |
| 139 然 |
Glucose (in DM25) |
| META TOPICMOVED |
by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli" |
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