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META TOPICPARENT |
name="ProtocolList" |
Isolating Phage Genomic DNA
This protocol has been tested with phage T7. It has a double-stranded genome that has a length of 40 kb.
Materials
- 5× phage precipitation solution (20% w/v PEG 8000, 2.5 M NaCl).
- 1× phage resuspension buffer (1M NaCl, 10 mM Tris•HCl pH 7.5, 0.1 mM EDTA).
- 10× NEB DNase I buffer (10 mM Tris-•HCl pH 7.6, 2.5 mM MgCl2, 0.5 mM CaCl2)
- DNase I (2,000 U/mL) (New England Biolabs M0303)
- RNase A (20 mg/mL) (Invitrogen PureLink™ RNase A #12091021)
- 0.5 M EDTA pH 8.0
- phenol:chloroform:isoamyl alcohol (25:24:1)
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