Golden Gate Assembly is a molecular cloning method used to recombine multiple DNA components into a single linear piece or plasmid. It bears similarity to restriction cloning by using a restriction endonuclease to produce "sticky ends", allowing for two terminal ends of DNA (either two distinct DNA molecules or a single DNA linear DNA molecule circularizing) to hybridize and be annealed together via a DNA Ligase.

To perform Golden Gate Assembly, one or more Type IIS Restriction Enzymes are used. Commonly used enzymes in the Barrick Lab are:

* BsmbI * BsaI * PaqCI

Reagents

* Your Restriction Enzyme(s) of choice * T4 ligase Buffer *