Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,004,251	T→C	intergenic (‑177/+425)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	1,004,251	0	T→C	100.0%	64.4	22	intergenic (‑177/+425)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (11/10):  ref base (0/0):  total (11/11)

TACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAG  >  REL606/1004218‑1004279                                  |                             tACCGTGAAAGTGCTACAAAGATAAAGATTTGGctt                            >  1:6103763/1‑36 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAAGATTTGGctt                            <  1:428326/36‑1 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAAGATTTGGctt                            >  1:4255336/1‑36 (MQ=255)  aCCGTGAAAGTGCTACAAAGATAAAGATTTGGCTTc                           >  1:4227892/1‑36 (MQ=255)   ccGTGAAAGTGCTACAAAGATAAAGATTTGGCTTCa                          <  1:6718059/36‑1 (MQ=255)   ccGTGAAAGTGCTACAAAGATAAAGATTTAGCTTCa                          <  1:2089835/36‑1 (MQ=25)    cGTGAAAGTGCTACAAAGATAAAGATTTGGCTTCaa                         <  1:1608325/36‑1 (MQ=255)    cGTGAAAGTGCTACAAAGATAAAGATTTGGCTTCaa                         >  1:1963964/1‑36 (MQ=255)    cGTGAAAGTGCTACAAAGATAAAGATTTGGCTTCaa                         >  1:5604522/1‑36 (MQ=255)      tGAAAGTGCTACAAAGATAAAGATTTGGCTTCaaaa                       >  1:7279698/1‑36 (MQ=255)       gAAAGTGCTACAAAGATAANGNTTTGGCTTCaaaaa                      <  1:1777435/36‑1 (MQ=255)       gAAAGTGCTACAAAGATAAAGATTTGGCTTCtaaaa                      >  1:1512685/1‑36 (MQ=25)       gAAAGTGCTACAAAGATAAAGATTTGGCTTCaaaaa                      >  1:3844092/1‑36 (MQ=255)           gTGCTACAAAGATAAAGATTTGGCTTCAAAAAGaaa                  <  1:2892584/36‑1 (MQ=255)                aCAAAGATAAAGATTTGGCTTCAAAAAGAAAAatat             >  1:5878507/1‑36 (MQ=255)                 cAAAGATAAAGATTTGGATTCAAAAAGAAAAATATg            <  1:5572824/36‑1 (MQ=255)                   aagataaagatTTGGCTTCAAAAAGAAAAATATGTa          <  1:6600838/36‑1 (MQ=255)                    agataaagatTTGGCTTCAAAAAGAAAAATATGTaa         <  1:2164548/36‑1 (MQ=255)                     gataaagatTTGGCTTCAAAAAGAAAAATATGTAAc        <  1:4941988/36‑1 (MQ=255)                      ataaagatTTGGCTTCAAAAAGAAAAATATGTAAcc       >  1:6336779/1‑36 (MQ=255)                         aagatTGGGCTTCAAAAAGAAAAATATGTAACCaaa    >  1:6521277/1‑36 (MQ=25)                          agatTTGGCTTCAAAAAGAAAAATATGTAACCaaaa   <  1:5080830/36‑1 (MQ=255)                           gatTTGGCTTCAAAAAGAAAAATATGTAACCaaaaa  <  1:2456287/36‑2 (MQ=255)                           gatTTGGCTTCAAAAAGAAAAATATGTAACCAAAAg  >  1:3448894/1‑36 (MQ=255)                           gatTTGGCTTCAAAAAGAAAAATATGTAACCAAAAg  >  1:1178388/1‑36 (MQ=255)                                  |                             TACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAG  >  REL606/1004218‑1004279 Base quality scores:  ATCG  < 3 ≤  ATCG  < 13 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 32 ≤  ATCG