Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,628,0270T→G25.0% 10.656N147T (AAC→ACC) arcADNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  new base (14/0):  ref base (18/24):  total (32/24)

TCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCT  >  REL606/4627997‑4628061
                              |                                  
tCGCCATCAGGGCCGATCAACGAACGGCTGGTggtt                               >  1:663866/1‑33 (MQ=25)
 cGCCATCAGGGCCGATCAACGAACGGCTGTTGATGt                              <  1:86101/36‑1 (MQ=255)
 cGCCATCAGGGCCGATCAACGAACGGCTGTGGGTGt                              >  1:4337029/1‑36 (MQ=25)
 cGCCATCAGGGCCGATCAACGAACGGCAGGTTATGt                              >  1:2778736/1‑36 (MQ=21)
  gCCATCAGGGCCGATCAACGAACGGCTGTTGATGTc                             <  1:1368962/36‑1 (MQ=255)
   ccATCAGGGCCGATCAACGAACGGCTGTTGATGTcc                            <  1:5810823/36‑1 (MQ=255)
   ccATCAGGGCCGATCAACGAACGGCTGTTGATGTcc                            <  1:5029269/36‑1 (MQ=255)
    cATCAGGGCCGATCAACGAACGGCTGTTGCTGTctc                           >  1:5562613/1‑34 (MQ=25)
    cATCAGGGCCGATCAACGAACGGCTGTTGATGttcc                           >  1:5548796/1‑33 (MQ=255)
     aTCAGGGCCGATCAACGAACGGCTGTTGATGTCCAg                          <  1:1858348/36‑1 (MQ=255)
     aTCAGGGCCGATCAACGAACGGCTGTTGATGTCCAg                          <  1:5724120/36‑1 (MQ=255)
     aTCAGGGCCGATCAACGAACGGCTGGTGGTGTCCAg                          >  1:1643964/1‑36 (MQ=25)
      tCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGt                         <  1:5519984/36‑1 (MQ=255)
       cAGGGCCGATCAACGAACGGCTGTTGATGTCCAGtt                        <  1:452170/36‑1 (MQ=255)
       cAGGGCCGAGCAACGAACGGCTGGTGATGTCCAGtt                        >  1:1113748/1‑36 (MQ=25)
        aGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTc                       <  1:4311575/36‑1 (MQ=255)
          ggCCGATCAACGAACGGCTGGTGGTTTGCAGTTtcc                     >  1:6303977/1‑36 (MQ=12)
          ggCCGATCAACGAACGGCTGGTGGTGTGCCGTTtcc                     >  1:5975988/1‑36 (MQ=12)
           gCCGATCAACGAACGGCTGTCGTTTGCCCGTTcccc                    >  1:7431439/1‑35 (MQ=12)
            ccGATCAACGAACGGCTGGTGATGTTCAGTTcccca                   >  1:2908066/1‑34 (MQ=21)
             cGATCAACGAACGGCTGGTGATGTCCCGTTcccccc                  >  1:4509094/1‑33 (MQ=21)
             cGATCAACGAACGGCTGGTGATGTCCCGTTCCCCAc                  >  1:3261262/1‑36 (MQ=21)
              gATCAACGAACGGCTGTTGATGTCCAGTTCCCAAcc                 <  1:5504570/36‑1 (MQ=255)
              gATCAACGAACGGCTGGTGGTGTCTAGTTGCCCAca                 >  1:5336532/1‑35 (MQ=12)
              aaTCAACGAACGGCTGTTGATGTCCAGTTCCCAAcc                 <  1:868481/35‑1 (MQ=255)
               aTCAACGAACGGCTGTTGATGTCCAGTTCCCCACCa                >  1:5669657/1‑36 (MQ=255)
               aTCAACGAACGGCTGTTGATGTCCAGTTCCCAACCa                <  1:4564698/36‑1 (MQ=255)
               aTCAACGAACGGCTGTTGATCTCCATTTCCCAACCa                >  1:5178455/1‑36 (MQ=25)
               aTCAACGAACGGCTGGTGTTGTCCCCTTTCCACCCa                >  1:3046375/1‑36 (MQ=9)
               aTCAACGAACGGCTGGTGGTGTCCAGTTTCCACccc                >  1:4713122/1‑35 (MQ=18)
                tCAACGAACGGCTGTTGATGTCCAGTTCCCAACCAt               <  1:6133496/36‑1 (MQ=255)
                tCAACGAACGGCTGGTGGTGTCCCGTTTCCCACCAt               >  1:7359536/1‑36 (MQ=12)
                 cAACGAACGGCTGTTGATGTCCCGATCCCCACCact              >  1:3470229/1‑34 (MQ=21)
                 cAACGAACGGCTGTTGATGTCCAGCTCCCAACCAtt              >  1:3465000/1‑36 (MQ=255)
                 cAACGAACGGCTGTTGATATCCAGTTCCCAACCCtt              >  1:6180958/1‑36 (MQ=25)
                  aacggacgGCTGTTGATGTCCAGTTCCCAACCATTg             <  1:924392/36‑1 (MQ=255)
                  aacgaacgGCTGTTGATGTCCAGTTCCCAACCATTg             <  1:3012820/36‑1 (MQ=255)
                  aacgaacgGCTGTTGATGTCCACTTCCCAAACATTg             >  1:3443578/1‑36 (MQ=25)
                  aacgaacgGCTGGTGATGTTCAGTTCCCAACCATTg             >  1:7393377/1‑36 (MQ=25)
                    cgaacgGCTGTTGATGTCCAGTTCCCAACCATTGaa           <  1:3226770/36‑1 (MQ=255)
                     gaacgGCTGTTGATGTCCAGTTCCCAACCATTGAAc          >  1:4721755/1‑36 (MQ=255)
                      aacgGCTGTTGATGTCCAGTTCCCAACCATTGAACt         <  1:2411330/36‑1 (MQ=255)
                       acgGCTGTTGATGTCCAGTTCCCAACCATTGAACtt        <  1:2910917/36‑1 (MQ=255)
                       acgGCTGTTGATGTCCAGTTCCCAACCATTGAACtt        <  1:1260397/36‑1 (MQ=255)
                        cgGGTGTTGATGTCCAGTTCCCAACCATTGAACTTg       <  1:3591594/36‑1 (MQ=255)
                        cgGCTGTTGATGTCCCGTTCCCCACCCCAGAACttt       >  1:5615505/1‑35 (MQ=255)
                         ggCTGTTGATGTCCAGTTCCCAACCATTGAACTTGt      <  1:1071026/36‑1 (MQ=255)
                          gCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTa     <  1:5193699/36‑1 (MQ=255)
                          gCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTa     <  1:7327591/36‑1 (MQ=255)
                          gCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTa     <  1:1623627/36‑1 (MQ=255)
                           cTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAg    >  1:5784016/1‑36 (MQ=255)
                           cTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAg    >  1:4901157/1‑36 (MQ=255)
                           cTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAg    >  1:7472688/1‑36 (MQ=255)
                            tGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGc   >  1:4238718/1‑36 (MQ=255)
                            tGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGc   >  1:154790/1‑36 (MQ=255)
                             gTTGATGTCCAGTTCCCAACCATTGAACTCGTAGCt  >  1:486608/1‑36 (MQ=255)
                              |                                  
TCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCT  >  REL606/4627997‑4628061

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG