Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,462,9920G→T33.8% 100.1154S77I (AGT→ATT) yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/49):  ref base (30/66):  total (30/124)

Only 100 of 156 total aligned reads displayed.

TCTTATGGAATTACTGGTGGGAATGTTTTTTTTAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGCAATAT  >  REL606/4462958‑4463027
                                  |                                        
  ttATGGAATTACTGGTGGGAATGTTTTTTTTAGTTg                                       <  1:3479190/36‑1 (MQ=255)
    aTGGAATTACTGGTGGGAATGTTTTTTTTAGTTG.Tc                                    >  1:5465227/1‑36 (MQ=255)
    aTGGAATTACTGGTGGGAATGTTTTTTTTAGTTG.Tc                                    >  1:6229027/1‑36 (MQ=255)
     tGGAATTACTGGTGGGAATGTTTTTTTTAGTTG.TC.t                                  <  1:6428469/36‑1 (MQ=255)
      ggAATTACTGGTGGGAATGTTTTTTTTAGTTG.TC.tt                                 >  1:5444602/1‑36 (MQ=255)
        aaTTACTGGTGGGAATGTTTTTTTTAGTTG.TC.tt.g.t                             <  1:2088294/36‑1 (MQ=255)
        aaTTACTGGTGGGAATGTTTTTTTTAGTTG.TC.tt.g.t                             <  1:3261198/36‑1 (MQ=255)
          ttACTGGCGGGAATGTTTTTTTTAGTTG.TC.tt.t.ttt                           >  1:4657543/1‑36 (MQ=25)
            aCTGGTGGGAATGTTTTTTTTAGTTG.TC.tc.t.ttttt                         >  1:1484458/1‑36 (MQ=25)
             cTGGTGGGAATGTTTTTTTTAGTTG.TC.tt.g.tttttg                        <  1:7007153/36‑1 (MQ=255)
             cTGGTGGGAATGTTTTTTTTAGTTG.TC.tt.g.ttttag                        >  1:1564819/1‑34 (MQ=255)
              tggtggGAATGTTTTTTTTAGTTG.TT.tt.g.tttttgt                       >  1:453135/1‑36 (MQ=255)
              tggtggGAATGTTTTTTTTAGTTG.TC.tt.g.tttttgt                       >  1:2521315/1‑36 (MQ=255)
               ggtggGAATGTTTTTTTTAGTTG.TC.tt.g.tttttgtt                      >  1:4706929/1‑36 (MQ=255)
               ggtggGAATGTTTTTGTTAGTTG.TC.tt.g.tttttgtt                      <  1:6356113/36‑1 (MQ=255)
                gtggGAATGTTTTTTTTAGTTG.TC.tt.g.tttttgttt                     >  1:5324622/1‑36 (MQ=255)
                 tggGAATGTTTTTTTTAGTTG.TC.tt.g.tttttgttct                    <  1:5667515/36‑3 (MQ=255)
                 tggGAATGTTTTTTTTAGTTG.TC.Tt.g.tttttgtttt                    >  1:3786295/1‑36 (MQ=255)
                    ttgtGTTTGTTTTGTTTT.TT.TT.T.TTGTTTtttt.ttt                <  1:6953552/33‑1 (MQ=2)
                    gtgtGTTTTTTTTCTTTT.TT.TT.T.GTTTTTtttt.ttt                <  1:3054908/33‑1 (MQ=2)
                    atgtGTGTTTTTTTTTTT.TT.TT.G.TTTTTTtttt.ttt                <  1:3668999/33‑1 (MQ=2)
                     tttGTCTTTTTTTTTTT.TG.TT.T.TTTTTTTTTT.TTTc               <  1:6866358/34‑1 (MQ=9)
                     tgtGTTTTTATCTGTTG.TT.TTCG.TTTTTGtttt.ttt                <  1:7384049/34‑1 (MQ=2)
                     tgtGTTTGTTTTGTTTT.TG.TT.T.TTTTTTtttt.tttt               <  1:6827770/34‑2 (MQ=9)
                     tctGTTTTTTCTTTTTT.TT.TT.T.TTTTTGTTTT.TTTc               <  1:2720065/34‑1 (MQ=2)
                     tcggTTTTTTTGTGTTT.TT.TG.T.TTTTTGTTTT.TTTc               <  1:3561262/33‑1 (MQ=2)
                     gttGTTTTTGTTTTTTT.TT.TT.G.TTTTTTtttt.tttt               <  1:6583772/34‑2 (MQ=2)
                     gaTGTTTTTTTTAGTTG.TC.TT.G.TTTTTGTTTT.TTTc               <  1:834183/35‑1 (MQ=255)
                     cgtGTCTTTTTTTGTTT.TT.TT.G.TTTTTTtttt.tttt               <  1:6510358/34‑2 (MQ=0)
                     aaTGTTTTTTTTAGTTG.TC.TT.G.TTTTTGtttt.tttt               >  1:2248449/1‑35 (MQ=255)
                      ttGTTTCTTTTGGTGT.TT.TT.T.TTTTTGtttt.ttttt              <  1:6768554/35‑3 (MQ=2)
                      ttGTGTTTTTTTTTTT.TG.TT.T.TTTTTTTTTT.TTTGt              <  1:7115022/35‑1 (MQ=9)
                      ttGTGTTTTTTGTTTT.TT.TT.T.TTTTTGtttt.ttttt              <  1:7264220/35‑3 (MQ=2)
                      gtGTTTTGTTTATTTG.TT.TTCT.TTTTTGtttt.tttt               <  1:7365761/35‑2 (MQ=2)
                      gtGTTTTCTTTTGTTT.TT.TT.GTTTTTTGtttt.tttt               <  1:6856863/35‑2 (MQ=0)
                      aTGTTTTTTTTAGTTG.TC.TT.G.TTTTTGtttt.ttttt              >  1:373022/1‑34 (MQ=255)
                       tcttttttttGTTTT.TT.TT.G.TTTTTTTTTTATTTCt              <  1:7237157/34‑1 (MQ=0)
                       tGTTTTTTTTTTTTT.TA.TT.G.TTTTTTtgtt.tttttt             <  1:6953773/36‑4 (MQ=0)
                       tGTTTTTTTTGTTTT.TT.TT.G.TTTTTTTTTT.TTTCtt             <  1:7300424/36‑2 (MQ=9)
                       tGTTTTTTTATTTTT.TT.TT.GTTTTTTGtttt.ttttt              <  1:7301630/36‑3 (MQ=2)
                       tGTTTTTTGTTGTTT.TT.TT.T.GTTTTTtttt.tttgtt             <  1:7200078/36‑4 (MQ=2)
                       tGTTTTGTTTTGTT..TT.TT.G.TTTTT.TTTT.TTTCTTtt           <  1:7373668/36‑1 (MQ=2)
                       tGTTTGTTTTTGTTT.TT.TT.G.TTTTTTtttt.ttttgt             <  1:6660208/36‑4 (MQ=0)
                       tGTCTTTTTTTTTTT.TT.TT.T.TTTTTGtttt.ttttct             <  1:6982795/36‑4 (MQ=2)
                        tttttttttGTTTT.TG.TT.T.TGTTTGTTTT.TTTTTTt            <  1:4238264/35‑1 (MQ=2)
                        tttttttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCttt            >  1:7467831/2‑34 (MQ=255)
                        ttttttttgTGTTGTTG.TT.T.TTTTTGTTTT.TTTCtt             <  1:7320185/35‑2 (MQ=2)
                        tttttttctTTTTG.TT.TT.GTTTTTTGTTTT.TTTCtt             <  1:7030189/35‑2 (MQ=0)
                        gTTTTTTTTAGTTG.TC.TT.G.TTTTTGTTTT.TTTCttt            >  1:311559/1‑34 (MQ=255)
                        gTTTTTTTTAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTAt            <  1:6237629/36‑1 (MQ=255)
                        gTTTTTTGTTTTTT.TG.TT.T.TTTTTTTTTT.TTGCttt            <  1:7320080/36‑3 (MQ=2)
                         ttttttttTGTTC.TT.TT.A.TTTTTTTGTT.TTTTTTtt           <  1:7310586/36‑1 (MQ=2)
                         ttttttttGTTTG.TT.TT.T.GTTTTTTTTT.TTGCtttc           <  1:7347477/36‑4 (MQ=2)
                         tttattttTGTTT.TT.TT.T.TGTTTTTTTT.TTTGTTtt           <  1:6580355/36‑1 (MQ=9)
                         ttctttttGTTTT.TC.TT.T.TTTTTTTGTT.TTTTTTtt           <  1:7200181/33‑1 (MQ=0)
                         tgttttttA.TTC.TC.TT.T.TTGTTTTTTT.TTTTTTttt          <  1:7011272/34‑1 (MQ=9)
                         ctttttttTTTTG.T..TT.A.TTTTTGTTTT.TTTTTTttt          <  1:6477284/35‑1 (MQ=2)
                          tttttttTTTTG..C.TT.T.TTTTTTTTTT.TTTCTAtttt         <  1:6973006/36‑2 (MQ=9)
                          tttttttTGTT..TT.TT.G.TTTTT.TTTT.TTTGTAttttt        <  1:6480639/36‑3 (MQ=0)
                          tttttttTCTTG.TT.TT.T.GTTTTTTCTT.TTTTTTttt          <  1:6510252/36‑1 (MQ=9)
                          tttttttGTTTT.TG.TT.G.TTTTTTTTTT.GTTTTTttt          <  1:6490092/36‑1 (MQ=2)
                          tttttttGTTTA.TT.TT.G.TTTTTGTTTT.TTTTTTttt          <  1:6469532/36‑1 (MQ=9)
                          tttttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTTTAttt          >  1:7135059/1‑36 (MQ=255)
                          tttttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTAttt          >  1:4579394/1‑36 (MQ=255)
                          tttttttA.TTT.TG.TT.T.TTTTCGTTTC.TTTTTTtttt         <  1:6992331/36‑2 (MQ=0)
                          ttttgttTGTTG.TT.TT.T.TGTTTTTTTT.TTTTTTttt          <  1:3862874/36‑1 (MQ=0)
                          tttatttGGTTT.TT.TT.G.TTTTTTTTTT.TTTTTTttt          <  1:3479249/36‑1 (MQ=9)
                          ttgttttTGTTC.TT.TT.T.TTTTTGTTTT.TTTTTTttt          <  1:3368036/33‑1 (MQ=2)
                          tgtttttTGTTG.TT.TT.T.TTTTTGTTTT.TTTTTTttt          <  1:3570334/34‑1 (MQ=2)
                          gttttttTTTTG.TT.GT.T.TTTTTGTTTT.TTTTTTttt          <  1:6458944/35‑1 (MQ=2)
                          gttttttGTTTT.TT.TT.G.TTTTTTTTTT.ATTTTTttt          <  1:6905235/35‑1 (MQ=2)
                          gttttttGGTTT.TT.TT.T.TTTTTGTTTT.TTTTTTttt          <  1:7328214/35‑1 (MQ=0)
                          gttttctAGTTG....TT.G.TTTTTGTTTT.TTTTTTttttt        <  1:6924536/35‑3 (MQ=2)
                          gtttattTGTTT.TT.TT.T.TTGTTTTTTT.TTTTTTttt          <  1:7143998/35‑1 (MQ=0)
                          cttttttTTTTT.TT.TT.G.TTTTTTTTTT.GTTCTTttt          <  1:7115177/35‑1 (MQ=2)
                           ttttttTTTTC.TG.TT.C.TTTTTGTTTT.TTTTTCtttt         <  1:6895488/36‑2 (MQ=0)
                           ttttttTGTTT.TG.TG.T.TTTTTGTTTT.TTTTTTtttt         <  1:7181282/36‑2 (MQ=2)
                           ttttttTCTTG.TC.TT...TTTTTGTTTT.TTTTTTttttt        <  1:6798474/36‑3 (MQ=0)
                           ttttttA.TTT.TT.TT.T.TTTTTGTTTT.TTTTTTtttgg        <  1:6640510/36‑3 (MQ=9)
                           tttttcTGTTT.TT.TT.G.TTTTTTTTTC.TTTTTAttgt         <  1:6768905/36‑3 (MQ=2)
                           tttttcTGTTG.TT.TT.G.TTTTTTTGTT.TTTTTTtttt         <  1:6580383/36‑2 (MQ=9)
                           gtttttTTTTG.TT.TT.A.TTTTCTTTTT.TGTTTTtttg         <  1:6924677/35‑2 (MQ=2)
                           gattttTGTTT.TT.TT.G.TTTTTTTTTT.TTTTTTtttt         <  1:7456279/34‑2 (MQ=9)
                            tttttTGTTG.TT.TG.T.TTTTTGTGTT.TTTGTTttttt        <  1:6699914/36‑3 (MQ=2)
                            tttttGGTTG.TT.TT.T.GTTTTCTTTT.TTCCTTttttt        <  1:6689322/36‑3 (MQ=0)
                            tttttCTTTG.TCTTT.G.TTTTTTTTTT.GTTTTTtttt         <  1:6529126/36‑2 (MQ=0)
                            tttttATTTG.TT.GT.T.TTTTTGTTTT.TTTTTTttttt        <  1:7039604/36‑3 (MQ=2)
                            tttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTTTTTct        >  1:4688369/1‑35 (MQ=255)
                            tttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTct        >  1:2475464/1‑35 (MQ=255)
                            tttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTct        >  1:7049367/1‑35 (MQ=255)
                            gttttAATTT.TT.TT.T.TTGTTTTTTT.TTTTTTTTTct        <  1:6837370/35‑2 (MQ=9)
                             ttttTGTTG.TTTTT.G.TTTTTTTTTT.TTTATTttttt        <  1:6600332/36‑3 (MQ=2)
                             ttttTGTTC.TT.TT.T.TTTTTTTGTT.TTTTTTTTTctt       <  1:7255717/36‑3 (MQ=2)
                             ttttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGc       <  1:257317/36‑1 (MQ=255)
                              tttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGCa      <  1:5024875/36‑1 (MQ=255)
                               ttAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCTCaa     >  1:7385047/1‑36 (MQ=255)
                                tAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGCAat    <  1:4099861/36‑1 (MQ=255)
                                 aGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCTCAata   >  1:7178448/1‑36 (MQ=255)
                                 aGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGCAata   <  1:3901407/36‑1 (MQ=255)
                                  gTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGCAatat  <  1:2821673/36‑1 (MQ=255)
                                  |                                        
TCTTATGGAATTACTGGTGGGAATGTTTTTTTTAGTTG.TC.TT.G.TTTTTGTTTT.TTTCTATTTCGCAATAT  >  REL606/4462958‑4463027

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 11 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG