Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,602,1010C→T100.0% 2.1 3intergenic (+124/+104)pinQ/ECB_01505predicted site‑specific recombinase/conserved hypothetical protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (0/1):  total (2/1)

TTACCCTACGGCAGTGCTGGCCATTCAATATCCTGTGCAGTTGACGTATCAACACGGTTCAGCAAC  >  REL606/1602070‑1602135
                               |                                  
ttACCCTACGGCAGTGCTGGCCATTCTATATTCttt                                >  1:5275201/1‑34 (MQ=1)
    ccTACGGCAGTGCTGGCCCTTTAATATTCTTTTCAg                            >  1:202760/1‑36 (MQ=0)
                              tCCTGTGCAGTTGACGTATCAACACGGTTCAGCAac  <  1:3105513/36‑1 (MQ=32)
                               |                                  
TTACCCTACGGCAGTGCTGGCCATTCAATATCCTGTGCAGTTGACGTATCAACACGGTTCAGCAAC  >  REL606/1602070‑1602135

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 8 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG