Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,545,1160G→T100.0% 0.9 2A294E (GCG→GAG) gadBglutamate decarboxylase B, PLP‑dependent
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/1):  ref base (1/0):  total (1/1)

GGTAATCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTC  >  REL606/1545081‑1545120
                                   |    
ggTAATCAACGTTGAACACCAGTTCCTGCGGCAgcg      >  1:1853749/1‑36 (MQ=38)
  tAATCAACGTTGAACACCAGTTCCTGCGGCAGCTct    <  1:950258/36‑1 (MQ=255)
    aTCAACGTTGAACACCAGTTCCTGCGGCAGCGcttc  >  1:7288346/1‑36 (MQ=32)
                                   |    
GGTAATCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTC  >  REL606/1545081‑1545120

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 32 ≤  ATCG