Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,536,7740G→T25.0% 11.057Q81K (CAA→AAA) ddpXD‑ala‑D‑ala dipeptidase, Zn‑dependent
Reads supporting (aligned to +/- strand):  new base (14/0):  ref base (9/33):  total (24/33)

CACATCAACAACATATTGCGGGTCTGGGCAGGCTTGCCACAACATCGCCTGTGCTTGTTGTGGGCGATAC  >  REL606/1536739‑1536808
                                   |                                  
cacaTCAACAACATATTGCGGGTCTGGGCAGGCTTg                                    <  1:3459348/36‑1 (MQ=255)
cacaTCAACAACATATTGCGGGTCTGGGCAGGCTTg                                    <  1:193469/36‑1 (MQ=255)
  caTCAACAGCATATTGCGGGTCTGGGCAGGCTTGcc                                  <  1:3647760/36‑1 (MQ=255)
  caTCAACAACATATTGCGGGTCTGGGCAGGCTTGcc                                  <  1:2460156/36‑1 (MQ=255)
  caTCAACAACATATTGCGGGTCTGGGCAGGCTTGcc                                  <  1:5710156/36‑1 (MQ=255)
   aTCAGCGACATATTGCGGGTCTGGGCAGGCTTGCca                                 <  1:1731435/36‑1 (MQ=25)
   aTCAACAACATATTGCGGGTCTGGGCAGGCTTgtcc                                 >  1:6472967/1‑33 (MQ=255)
   aTCAACAACATATTGCGGGTCTGGGCAGGCTTTccc                                 >  1:3220610/1‑35 (MQ=255)
     caacaaCATATTGCGGGTCTGGGCAGGCTTTcccaa                               >  1:5952123/1‑33 (MQ=25)
     caacaaCATATTGCGGGTCTGGGCAGGCTTGCcaca                               <  1:6405085/36‑1 (MQ=255)
      aacaacATATTGCGGGTCTGGGCAGGCTTGCCacaa                              <  1:3227039/36‑1 (MQ=255)
      aacaacATATTGCGGGTCTGGGCAGGCTTGCCacaa                              <  1:1057953/36‑1 (MQ=255)
       acaacaTATTGCGGGTCTGGGCAGGCTTTCCacaac                             >  1:5710381/1‑36 (MQ=255)
       acaacaTATTGCGGGTCTGGGCAGGCTTGCCacaac                             <  1:7459025/36‑1 (MQ=255)
        caacaTATTGCGGGTCTGGGCAGGCTTTCCacaaca                            >  1:5034401/1‑36 (MQ=255)
        caacaTATTGCGGGTCTGGGCAGGCTTGCCacaaca                            <  1:1190351/36‑1 (MQ=255)
          acaTATTGCGGGTCTGGGCAGGCTTTCTccaacaac                          >  1:6893621/1‑34 (MQ=18)
           caaatTGCGGGTCTGGGCAGGCTTGCCACAACATCg                         <  1:3592781/33‑1 (MQ=255)
           caTATTGCGGGTCTGGGCAGGCTTTTCACAACATCg                         >  1:3667146/1‑36 (MQ=25)
           caTATTGCGGGTCTGGGCAGGCTTGCCACAACATCg                         <  1:7111212/36‑1 (MQ=255)
            atatTGCTGGTCTGGGCAGGCTTGTAACAAAATctc                        >  1:851402/1‑34 (MQ=17)
            atatTGCGGGTCTGGGCAGGCTTTCCACAACATccc                        >  1:1560358/1‑34 (MQ=25)
              atTGCGGGTCTGGGCAGGCTTTTCACCACAACGccc                      >  1:1996932/1‑35 (MQ=18)
              atTGCGGGTCTGGGCAGGCTTTTCAAAAAATAGCCt                      >  1:1284062/1‑36 (MQ=12)
              atTGCGGGTCTGGGCAGGCTTGCCACAACATCGCCt                      >  1:958193/1‑36 (MQ=255)
                tgggggTCTGGGCAGGCTTGCCACAACATCGCCtgt                    <  1:1507623/33‑1 (MQ=255)
                tGCGGGCCTGGGCAGGCTTGCCACAACATCGCCtgt                    <  1:6315408/36‑1 (MQ=255)
                 gCGGGTCTGGGCAGGCTTGCCACAACATCGCCtgtg                   <  1:6947751/36‑1 (MQ=255)
                 gCGGGTCTGGGCAGGCTTGCCACAACATCGCCtgtg                   <  1:1843191/36‑1 (MQ=255)
                 gCGGGTCTGGGCAGGCTTGCCACAACATCGCCtgtg                   <  1:4852819/36‑1 (MQ=255)
                 gCGGGCCTGGGCAGGCTTGCCACAACATCGCCtgtg                   <  1:6295314/36‑1 (MQ=255)
                  cGGGTCTGGGCAGGCTTACCACAACATCGCCTGTGc                  >  1:7211215/1‑36 (MQ=255)
                   gggTCTGGGCAGGCTTGCCACAACATCGCCTGTGCt                 <  1:2972629/36‑1 (MQ=255)
                    ggTCTGGGCAGGCTTTCCCCAACATCCCCTTTGGtt                >  1:2456573/1‑36 (MQ=12)
                    ggTCTGGGCAGGCTTTCCACAACATCCCCCGCGCtt                >  1:905792/1‑36 (MQ=18)
                    ggTCTGGGCAGGCTTGCCACAACATCGCCTGTGCtt                <  1:6365198/36‑1 (MQ=255)
                     gTCTGGGCAGGCTTTCCACCACATCGCCTGTCCtcg               >  1:2245758/1‑34 (MQ=18)
                      tgtGGGGAGGGTTGCCACAACATCGCCTGTGCttgt              <  1:3696826/34‑1 (MQ=25)
                      tCTGGGCAGGCTTTCCCCAAAATCCCCTGTGCtttt              >  1:3736770/1‑34 (MQ=17)
                        ttggCAGGCTTGCCACAACATCGCCTGTGCttgttg            <  1:1691391/34‑1 (MQ=255)
                        tGGGCGGGTTTGCAACAACATCGCCTGTGCttgttg            <  1:3614563/36‑1 (MQ=21)
                        tGGGCAGGCTTGCCACCACATCCCCCGTGCttgttt            >  1:4310005/1‑35 (MQ=21)
                        ggggCTGACTTGCCACAACATCGCCGGTGCttgttg            <  1:4874016/35‑1 (MQ=18)
                         gggCAGGCTTTCCACAACATCGCCTGTGCttgtttt           >  1:6052380/1‑34 (MQ=25)
                          ggCAGGCTTGCCACAACATCGCCTGTGCTTGTtgtg          <  1:471155/36‑1 (MQ=255)
                            cAGGCTTGCCACAACATCGCCTGTGCTTGTTGTggg        >  1:5718271/1‑36 (MQ=255)
                            cAGGCTTGCCACAACATCGCCTGTGCTTCTTGTggg        >  1:5775773/1‑36 (MQ=255)
                             aGGCTTGCCACAACATCGCCTGTGCTTGTTGTGGGc       <  1:7139924/36‑1 (MQ=255)
                              ggCTTGCCCCAACATCGCCTCTGCTTGTTGTGGGCg      >  1:5119035/1‑36 (MQ=25)
                              ggCTTGCCACAACATCGCCTGTGGTTGTTTTggggg      >  1:6727868/1‑34 (MQ=25)
                              ggCTTGCCACAACATCGCCTGTGCTTGTTGTGGGCg      <  1:3459526/36‑1 (MQ=255)
                                cTTGCCGCAACATCGCCTGTGCTTGTTGTGGGCGAt    <  1:1177844/36‑1 (MQ=255)
                                cTTGCCACAACATCGCCTGTGCTTGTTGTGGGCGAt    <  1:1701089/36‑1 (MQ=255)
                                 ttGCCACAACATCGCCTGTGCTTGTTGTGGGCGATa   >  1:2064726/1‑36 (MQ=255)
                                 ttGCCACAACATCGCCTGTGCTTGTTGTGGGCGATa   <  1:1321671/36‑1 (MQ=255)
                                 ttGCCAAAACATCGCCTGTGCTTGTTGTGGGCGATa   <  1:114061/36‑1 (MQ=255)
                                  tGCCACATCATCGCCTGTGCTTGTTGTGGGCGATAc  <  1:3271880/36‑1 (MQ=255)
                                  tGCCACAACATCGCCTGTGCTTGTTGTGGGCGATAc  >  1:885399/1‑36 (MQ=255)
                                  tGCCACAACATCGCCTGTGCTTGTTGTGGGCGATAc  <  1:6128064/36‑1 (MQ=255)
                                  tGCCACAACATCGCCTGTGCTTGTTGTGGGCGATAc  <  1:939632/36‑1 (MQ=255)
                                   |                                  
CACATCAACAACATATTGCGGGTCTGGGCAGGCTTGCCACAACATCGCCTGTGCTTGTTGTGGGCGATAC  >  REL606/1536739‑1536808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 11 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG