---+++ Dpn I digestion *Purpose* <br> To digest (Adeno) methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples. <br> *Procurement* <br> Can be ordered directly from NEB. <br> Typically stored at -20°C; can be found in the common enzyme freezer box. <br> *Use* <br> Use in NEBuffer 4; however, it can be added directly to PCR sample.<br> Incubate at 37°C for one hour or overnight. <br> Heat inactivate by incubating at 80°C for 20 minutes.<br> NEB unit definition: One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl. NEB buffer composition: 1X NEBuffer 4: <br> 20 mM Tris-acetate <br> 50 mM potassium acetate <br> 10 mM Magnesium Acetate <br> 1 mM Dithiothreitol <br> pH 7.9 @ 25°C <br>
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Topic revision: r1 - 2012-08-15 - CraigBarnhart