<noautolink> ---+ 16S rRNA Sequencing to Identify Unknown Microbes Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? ---++ PCR Reaction For PCR we use universal primers U341F and UA1406R that amplify an approximately 1 kb stretch of rDNA and should work for nearly any bacterial or archaeal sequence [1]. | *primer* | *sequence* | | U341F | CCTACGGGRSGCAGCAG | | UA1406R | ACGGGCGGTGWGTRCAA | ---++ Analysis Use the tools at the [[http://rdp.cme.msu.edu/][Ribosomal Protein Database]]. ---++ References 1. Baker, G.C., Smith, J.J. & Cowan, D.A. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55, 541-55 (2003).
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Topic revision: r1 - 2010-05-13 - JeffreyBarrick