---+ Protocol Updates Needed (as of 2016_Dec_15) ---++ Protocols needed or flagged for updates * Flow protocol updates (Dan and Dacia) * P1 transduction; FLP/FRT recombination (Dan) * Chemical Competent Cells and Zcomp cells(Colin and Peng?) * ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?) * ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab (<- Simon?) * NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header? (<- Simon?) * Update Gibson Assembly protocol - add images for clarity and future-proof language (location) ---++ Recently finished protocols (kept until next Hackathon) * ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions <- Included in "working with non-canonical amino acids" section * ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells. <- I updated EC protocol to include a large batch option, and split the transformation to a new page. Could still use pictures on both protocols. * ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid. (finished!) * AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!) * ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote a paper about this....) * LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)
This topic: Lab
>
WebHome
>
ProtocolList
>
ProtocolUpdatesNeeded
Topic revision: r21 - 2016-12-15 - SeanLeonard