---+ _topA_ Gene Sequencing The _topA_ gene coordinates are 1329420-1332017. The _topA_ mutation in the Ara-1 long-term line is 1329516:C→T. | *primer* | *who* | *coords* | *sequence* | | RL0398 | TC | 1329010-1329031 | 5'‑CGCGGTCGATGGGTTGTGTCTC | | RL0399 | TC | 1332121-1332101 | 5'‑ATCATTTATAGCCTTAGTGTA | | RL0400 | TC | 1329557-1329577 | 5'‑GAGTGCCGACTCTACCTCCAC | | RL0401 | TC | 1330088-1330109 | 5'‑TGCCAGCACGACCACGCCATCT | | RL0402 | TC | 1330609-1330629 | 5'‑AGTTCGTTGCCTGCCAGATGA | | RL0403 | TC | 1331103-1331122 | 5'‑GCTGTACTGGATCACTTCTT | | RL0410, RL0610 | RW, BW | 1329489-1329508 | 5'‑GACTACGTGGTGAAATCCAG | | RL0411, RL0611 | RW, BW | 1329683-1329663 | 5'‑ACCAGGCAACACTTCATAGTG | | RL0738 | CO | 1329010-1329029 | 5'‑CGCGGTCGATGGGTTGTGTC | | RL0739 | CO | 1332323-1332302 | 5'‑TCCCGGGTTGTGATGTGTAAAG | | RL0740 | CO | 1329648-1329670 | 5'‑CACAATTGGGAGGCGCACTATGA | | RL0741 | CO | 1331694-1331672 | 5'‑GTAATGGCACCGGATCTTCTTTC | | RL0742 | CO | 1329990-1330007 | 5'‑GCCTGTCTGCCGGTCGTGTG | | RL0743 | CO | 1331055-1331032* | 5'‑TCTGCGCGGTAAAgTCGTAGTTCA | * This primer has a base mismatch (lowercase) to REL606. It must have been designed based on the MG1655 sequence. All coordinates are for the reference strain REL606.
This topic: Lab
>
WebHome
>
ProtocolList
>
ProceduresTargetedSequencing
>
ProceduresSequencingTopA
Topic revision: r1 - 2009-08-05 - JeffreyBarrick