RNA Isolation

RNA Quality Check

At the CAFG, use the Agilent 2100 Bioanalyzer to check the quality of RNA samples. There are twelve samples per $30 chip. Select the "Bacterial RNA assay option". The ratio of the 16S RNA to 23S RNA peaks should be 1.9 in a high quality sample.

RNA Labeling

RNA Hybridization

Slide Scanning